Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_005222 and ABIE53_005223 are separated by 33 nucleotides ABIE53_005223 and ABIE53_005224 are separated by 8 nucleotides ABIE53_005224 and ABIE53_005225 are separated by 41 nucleotides
ABIE53_005222: ABIE53_005222 - diguanylate cyclase (GGDEF)-like protein, at 1,479,218 to 1,481,551
_005222
ABIE53_005223: ABIE53_005223 - 4-hydroxy-2-oxoheptanedioate aldolase, at 1,481,585 to 1,482,388
_005223
ABIE53_005224: ABIE53_005224 - 2-oxo-hept-3-ene-1,7-dioate hydratase, at 1,482,397 to 1,483,200
_005224
ABIE53_005225: ABIE53_005225 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,483,242 to 1,483,643
_005225
Position (kb)
1481
1482
1483 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1480.860 kb on + strand, within ABIE53_005222 at 1480.861 kb on - strand, within ABIE53_005222 at 1480.861 kb on - strand, within ABIE53_005222 at 1480.935 kb on + strand, within ABIE53_005222 at 1480.935 kb on + strand, within ABIE53_005222 at 1480.936 kb on - strand, within ABIE53_005222 at 1480.936 kb on - strand, within ABIE53_005222 at 1480.936 kb on - strand, within ABIE53_005222 at 1480.936 kb on - strand, within ABIE53_005222 at 1480.936 kb on - strand, within ABIE53_005222 at 1480.974 kb on + strand, within ABIE53_005222 at 1480.975 kb on - strand, within ABIE53_005222 at 1481.012 kb on + strand, within ABIE53_005222 at 1481.013 kb on - strand, within ABIE53_005222 at 1481.450 kb on - strand at 1481.587 kb on - strand at 1481.590 kb on - strand at 1481.632 kb on - strand at 1481.716 kb on - strand, within ABIE53_005223 at 1481.980 kb on - strand, within ABIE53_005223 at 1482.304 kb on - strand, within ABIE53_005223 at 1482.803 kb on + strand, within ABIE53_005224 at 1483.196 kb on - strand at 1483.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 1,480,860 + ABIE53_005222 0.70 -1.5 1,480,861 - ABIE53_005222 0.70 -0.2 1,480,861 - ABIE53_005222 0.70 -0.2 1,480,935 + ABIE53_005222 0.74 +0.7 1,480,935 + ABIE53_005222 0.74 +1.4 1,480,936 - ABIE53_005222 0.74 +1.3 1,480,936 - ABIE53_005222 0.74 +2.1 1,480,936 - ABIE53_005222 0.74 +0.6 1,480,936 - ABIE53_005222 0.74 +2.4 1,480,936 - ABIE53_005222 0.74 -0.3 1,480,974 + ABIE53_005222 0.75 -1.1 1,480,975 - ABIE53_005222 0.75 +0.8 1,481,012 + ABIE53_005222 0.77 -0.5 1,481,013 - ABIE53_005222 0.77 -0.8 1,481,450 - -1.8 1,481,587 - -1.7 1,481,590 - -0.1 1,481,632 - -0.3 1,481,716 - ABIE53_005223 0.16 -1.1 1,481,980 - ABIE53_005223 0.49 -0.9 1,482,304 - ABIE53_005223 0.89 -1.5 1,482,803 + ABIE53_005224 0.50 +0.1 1,483,196 - +0.8 1,483,211 + -2.0
Or see this region's nucleotide sequence