Experiment: MoYLS4 with Chloride 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0007 and DvMF_R0002 are separated by 71 nucleotides DvMF_R0002 and DvMF_R0003 are separated by 57 nucleotides DvMF_R0003 and DvMF_R0004 are separated by 8 nucleotides DvMF_R0004 and DvMF_R0005 are separated by 13 nucleotides DvMF_R0005 and DvMF_0008 are separated by 475 nucleotides DvMF_0008 and DvMF_0009 are separated by 205 nucleotides
DvMF_0007: DvMF_0007 - protein of unknown function DUF302 (RefSeq), at 9,037 to 9,444
_0007
DvMF_R0002: DvMF_R0002 - tRNA-Gly (RefSeq), at 9,516 to 9,590
_R0002
DvMF_R0003: DvMF_R0003 - tRNA-Gly (RefSeq), at 9,648 to 9,722
_R0003
DvMF_R0004: DvMF_R0004 - tRNA-Cys (RefSeq), at 9,731 to 9,805
_R0004
DvMF_R0005: DvMF_R0005 - tRNA-Gly (RefSeq), at 9,819 to 9,893
_R0005
DvMF_0008: DvMF_0008 - transcriptional regulator, TraR/DksA family (RefSeq), at 10,369 to 10,731
_0008
DvMF_0009: DvMF_0009 - protein of unknown function DUF814 (RefSeq), at 10,937 to 12,919
_0009
Position (kb) 10
11 Strain fitness (log2 ratio) -1
0
1 at 9.399 kb on - strand, within DvMF_0007 at 9.412 kb on + strand at 9.553 kb on - strand, within DvMF_R0002 at 9.702 kb on + strand, within DvMF_R0003 at 10.109 kb on + strand at 10.951 kb on + strand at 11.107 kb on + strand at 11.337 kb on + strand, within DvMF_0009 at 11.371 kb on - strand, within DvMF_0009 at 11.413 kb on - strand, within DvMF_0009
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Chloride 125 mM remove 9,399 - DvMF_0007 0.89 -0.7 9,412 + -0.7 9,553 - DvMF_R0002 0.49 -0.3 9,702 + DvMF_R0003 0.72 -0.4 10,109 + +0.0 10,951 + -0.4 11,107 + -0.2 11,337 + DvMF_0009 0.20 -0.8 11,371 - DvMF_0009 0.22 -0.4 11,413 - DvMF_0009 0.24 -0.6
Or see this region's nucleotide sequence