Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3542 and Shew_3543 are separated by 29 nucleotides Shew_3543 and Shew_3544 are separated by 148 nucleotides Shew_3544 and rbn overlap by 4 nucleotides
Shew_3542: Shew_3542 - D-tyrosyl-tRNA deacylase (RefSeq), at 4,230,502 to 4,230,939
_3542
Shew_3543: Shew_3543 - hypothetical protein (RefSeq), at 4,230,969 to 4,231,532
_3543
Shew_3544: Shew_3544 - alpha/beta hydrolase fold (RefSeq), at 4,231,681 to 4,232,631
_3544
Shew_3545: rbn - ribonuclease BN (RefSeq), at 4,232,628 to 4,233,485
rbn
Position (kb)
4231
4232
4233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4230.752 kb on - strand, within Shew_3542 at 4230.795 kb on + strand, within Shew_3542 at 4230.902 kb on + strand at 4231.145 kb on + strand, within Shew_3543 at 4231.223 kb on + strand, within Shew_3543 at 4231.231 kb on - strand, within Shew_3543 at 4231.231 kb on - strand, within Shew_3543 at 4231.231 kb on - strand, within Shew_3543 at 4231.317 kb on - strand, within Shew_3543 at 4231.447 kb on + strand, within Shew_3543 at 4232.002 kb on - strand, within Shew_3544 at 4232.031 kb on + strand, within Shew_3544 at 4232.170 kb on + strand, within Shew_3544 at 4232.170 kb on + strand, within Shew_3544 at 4232.189 kb on + strand, within Shew_3544 at 4232.287 kb on + strand, within Shew_3544 at 4232.295 kb on - strand, within Shew_3544 at 4232.408 kb on + strand, within Shew_3544 at 4232.521 kb on + strand, within Shew_3544 at 4232.932 kb on - strand, within rbn at 4233.053 kb on + strand, within rbn at 4233.099 kb on + strand, within rbn at 4233.235 kb on + strand, within rbn at 4233.235 kb on + strand, within rbn at 4233.243 kb on - strand, within rbn at 4233.271 kb on + strand, within rbn at 4233.371 kb on + strand, within rbn at 4233.379 kb on - strand, within rbn at 4233.416 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 4,230,752 - Shew_3542 0.57 -0.0 4,230,795 + Shew_3542 0.67 +1.7 4,230,902 + -0.3 4,231,145 + Shew_3543 0.31 +4.8 4,231,223 + Shew_3543 0.45 -3.2 4,231,231 - Shew_3543 0.46 -2.2 4,231,231 - Shew_3543 0.46 -2.5 4,231,231 - Shew_3543 0.46 -0.5 4,231,317 - Shew_3543 0.62 -2.2 4,231,447 + Shew_3543 0.85 -1.0 4,232,002 - Shew_3544 0.34 +0.8 4,232,031 + Shew_3544 0.37 -0.6 4,232,170 + Shew_3544 0.51 -0.2 4,232,170 + Shew_3544 0.51 +2.3 4,232,189 + Shew_3544 0.53 -1.2 4,232,287 + Shew_3544 0.64 +0.3 4,232,295 - Shew_3544 0.65 +0.9 4,232,408 + Shew_3544 0.76 +0.3 4,232,521 + Shew_3544 0.88 +0.9 4,232,932 - rbn Shew_3545 0.35 -0.1 4,233,053 + rbn Shew_3545 0.50 -3.0 4,233,099 + rbn Shew_3545 0.55 +0.2 4,233,235 + rbn Shew_3545 0.71 +0.5 4,233,235 + rbn Shew_3545 0.71 -1.9 4,233,243 - rbn Shew_3545 0.72 -3.2 4,233,271 + rbn Shew_3545 0.75 +1.3 4,233,371 + rbn Shew_3545 0.87 +3.2 4,233,379 - rbn Shew_3545 0.88 -2.5 4,233,416 - -0.9
Or see this region's nucleotide sequence