Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3345 and Shew_3346 are separated by 201 nucleotides Shew_3346 and Shew_3347 are separated by 2 nucleotides Shew_3347 and Shew_3348 are separated by 34 nucleotides
Shew_3345: Shew_3345 - lipid kinase (RefSeq), at 3,989,642 to 3,990,565
_3345
Shew_3346: Shew_3346 - hypothetical protein (RefSeq), at 3,990,767 to 3,991,450
_3346
Shew_3347: Shew_3347 - hypothetical protein (RefSeq), at 3,991,453 to 3,993,333
_3347
Shew_3348: Shew_3348 - pseudouridine synthase (RefSeq), at 3,993,368 to 3,994,309
_3348
Position (kb)
3991
3992
3993
3994 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3990.686 kb on - strand at 3990.710 kb on - strand at 3990.782 kb on - strand at 3990.937 kb on - strand, within Shew_3346 at 3990.976 kb on - strand, within Shew_3346 at 3991.092 kb on + strand, within Shew_3346 at 3991.100 kb on - strand, within Shew_3346 at 3991.326 kb on - strand, within Shew_3346 at 3991.561 kb on - strand at 3991.563 kb on + strand at 3991.793 kb on - strand, within Shew_3347 at 3992.219 kb on + strand, within Shew_3347 at 3992.486 kb on + strand, within Shew_3347 at 3992.559 kb on + strand, within Shew_3347 at 3992.567 kb on - strand, within Shew_3347 at 3992.615 kb on + strand, within Shew_3347 at 3992.623 kb on - strand, within Shew_3347 at 3993.344 kb on + strand at 3993.344 kb on + strand at 3993.352 kb on - strand at 3993.533 kb on - strand, within Shew_3348 at 3993.661 kb on + strand, within Shew_3348 at 3993.927 kb on - strand, within Shew_3348 at 3993.992 kb on + strand, within Shew_3348 at 3994.079 kb on - strand, within Shew_3348 at 3994.163 kb on + strand, within Shew_3348 at 3994.256 kb on + strand at 3994.264 kb on - strand at 3994.264 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,990,686 - -0.6 3,990,710 - -1.1 3,990,782 - -0.4 3,990,937 - Shew_3346 0.25 +1.4 3,990,976 - Shew_3346 0.31 -0.3 3,991,092 + Shew_3346 0.48 -0.2 3,991,100 - Shew_3346 0.49 -0.7 3,991,326 - Shew_3346 0.82 -0.9 3,991,561 - +0.6 3,991,563 + +1.5 3,991,793 - Shew_3347 0.18 +0.7 3,992,219 + Shew_3347 0.41 -0.2 3,992,486 + Shew_3347 0.55 +0.8 3,992,559 + Shew_3347 0.59 +1.3 3,992,567 - Shew_3347 0.59 -0.5 3,992,615 + Shew_3347 0.62 -3.1 3,992,623 - Shew_3347 0.62 +3.3 3,993,344 + +0.4 3,993,344 + +1.0 3,993,352 - +1.3 3,993,533 - Shew_3348 0.18 -0.1 3,993,661 + Shew_3348 0.31 -0.0 3,993,927 - Shew_3348 0.59 +0.4 3,993,992 + Shew_3348 0.66 -1.4 3,994,079 - Shew_3348 0.75 -0.4 3,994,163 + Shew_3348 0.84 +0.1 3,994,256 + -0.2 3,994,264 - -2.5 3,994,264 - +0.5
Or see this region's nucleotide sequence