Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3325 and Shew_3326 overlap by 4 nucleotides Shew_3326 and Shew_3327 are separated by 78 nucleotides
Shew_3325: Shew_3325 - major facilitator transporter (RefSeq), at 3,965,280 to 3,966,449
_3325
Shew_3326: Shew_3326 - sodium/hydrogen exchanger (RefSeq), at 3,966,446 to 3,967,687
_3326
Shew_3327: Shew_3327 - RNA-metabolising metallo-beta-lactamase (RefSeq), at 3,967,766 to 3,969,211
_3327
Position (kb)
3966
3967
3968 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3965.766 kb on + strand, within Shew_3325 at 3965.774 kb on - strand, within Shew_3325 at 3965.820 kb on + strand, within Shew_3325 at 3965.881 kb on + strand, within Shew_3325 at 3965.943 kb on + strand, within Shew_3325 at 3965.952 kb on - strand, within Shew_3325 at 3966.283 kb on + strand, within Shew_3325 at 3966.283 kb on + strand, within Shew_3325 at 3966.291 kb on - strand, within Shew_3325 at 3966.949 kb on - strand, within Shew_3326 at 3967.011 kb on - strand, within Shew_3326 at 3967.124 kb on - strand, within Shew_3326 at 3967.275 kb on + strand, within Shew_3326 at 3967.283 kb on - strand, within Shew_3326 at 3967.283 kb on - strand, within Shew_3326 at 3967.810 kb on + strand at 3967.810 kb on + strand at 3967.818 kb on - strand at 3968.035 kb on + strand, within Shew_3327 at 3968.370 kb on - strand, within Shew_3327 at 3968.514 kb on + strand, within Shew_3327 at 3968.514 kb on + strand, within Shew_3327 at 3968.522 kb on - strand, within Shew_3327 at 3968.556 kb on - strand, within Shew_3327 at 3968.605 kb on + strand, within Shew_3327
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,965,766 + Shew_3325 0.42 -4.2 3,965,774 - Shew_3325 0.42 +0.4 3,965,820 + Shew_3325 0.46 +0.2 3,965,881 + Shew_3325 0.51 -0.0 3,965,943 + Shew_3325 0.57 +0.1 3,965,952 - Shew_3325 0.57 -0.4 3,966,283 + Shew_3325 0.86 -0.2 3,966,283 + Shew_3325 0.86 -2.3 3,966,291 - Shew_3325 0.86 -0.9 3,966,949 - Shew_3326 0.40 -0.9 3,967,011 - Shew_3326 0.45 -0.6 3,967,124 - Shew_3326 0.55 -3.1 3,967,275 + Shew_3326 0.67 +0.8 3,967,283 - Shew_3326 0.67 -2.0 3,967,283 - Shew_3326 0.67 +0.6 3,967,810 + +0.1 3,967,810 + +0.0 3,967,818 - -1.3 3,968,035 + Shew_3327 0.19 +2.8 3,968,370 - Shew_3327 0.42 -0.2 3,968,514 + Shew_3327 0.52 -0.9 3,968,514 + Shew_3327 0.52 +2.0 3,968,522 - Shew_3327 0.52 -2.0 3,968,556 - Shew_3327 0.55 -0.9 3,968,605 + Shew_3327 0.58 +0.6
Or see this region's nucleotide sequence