Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3259 and Shew_3260 are separated by 537 nucleotides Shew_3260 and Shew_3261 are separated by 108 nucleotides Shew_3261 and Shew_3262 are separated by 105 nucleotides
Shew_3259: Shew_3259 - heavy metal sensor signal transduction histidine kinase (RefSeq), at 3,896,461 to 3,897,849
_3259
Shew_3260: Shew_3260 - porin (RefSeq), at 3,898,387 to 3,899,415
_3260
Shew_3261: Shew_3261 - thiopurine S-methyltransferase (RefSeq), at 3,899,524 to 3,900,180
_3261
Shew_3262: Shew_3262 - hypothetical protein (RefSeq), at 3,900,286 to 3,900,630
_3262
Position (kb)
3898
3899
3900 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3897.452 kb on + strand, within Shew_3259 at 3897.460 kb on + strand, within Shew_3259 at 3897.560 kb on - strand, within Shew_3259 at 3897.657 kb on + strand, within Shew_3259 at 3897.662 kb on + strand, within Shew_3259 at 3897.670 kb on - strand, within Shew_3259 at 3897.670 kb on - strand, within Shew_3259 at 3897.670 kb on - strand, within Shew_3259 at 3897.670 kb on - strand, within Shew_3259 at 3897.737 kb on + strand at 3897.742 kb on + strand at 3897.750 kb on - strand at 3897.750 kb on - strand at 3897.750 kb on - strand at 3897.751 kb on - strand at 3897.911 kb on - strand at 3898.429 kb on + strand at 3898.509 kb on + strand, within Shew_3260 at 3898.517 kb on - strand, within Shew_3260 at 3898.517 kb on - strand, within Shew_3260 at 3898.517 kb on - strand, within Shew_3260 at 3898.543 kb on + strand, within Shew_3260 at 3898.713 kb on - strand, within Shew_3260 at 3899.114 kb on + strand, within Shew_3260 at 3899.165 kb on + strand, within Shew_3260 at 3899.165 kb on + strand, within Shew_3260 at 3899.173 kb on - strand, within Shew_3260 at 3899.182 kb on + strand, within Shew_3260 at 3899.392 kb on - strand at 3899.710 kb on + strand, within Shew_3261 at 3899.860 kb on + strand, within Shew_3261 at 3899.860 kb on + strand, within Shew_3261 at 3899.879 kb on + strand, within Shew_3261 at 3900.228 kb on - strand at 3900.278 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,897,452 + Shew_3259 0.71 -1.7 3,897,460 + Shew_3259 0.72 -1.0 3,897,560 - Shew_3259 0.79 -1.0 3,897,657 + Shew_3259 0.86 -2.2 3,897,662 + Shew_3259 0.86 +0.1 3,897,670 - Shew_3259 0.87 -1.1 3,897,670 - Shew_3259 0.87 +0.8 3,897,670 - Shew_3259 0.87 -3.9 3,897,670 - Shew_3259 0.87 -1.9 3,897,737 + -1.6 3,897,742 + -1.2 3,897,750 - -0.8 3,897,750 - -1.9 3,897,750 - -0.5 3,897,751 - -1.0 3,897,911 - -2.5 3,898,429 + -3.5 3,898,509 + Shew_3260 0.12 -4.0 3,898,517 - Shew_3260 0.13 -0.3 3,898,517 - Shew_3260 0.13 -2.6 3,898,517 - Shew_3260 0.13 -4.2 3,898,543 + Shew_3260 0.15 -3.1 3,898,713 - Shew_3260 0.32 -0.4 3,899,114 + Shew_3260 0.71 -2.1 3,899,165 + Shew_3260 0.76 -3.0 3,899,165 + Shew_3260 0.76 -4.2 3,899,173 - Shew_3260 0.76 -3.3 3,899,182 + Shew_3260 0.77 -3.8 3,899,392 - -1.8 3,899,710 + Shew_3261 0.28 -0.9 3,899,860 + Shew_3261 0.51 +0.9 3,899,860 + Shew_3261 0.51 -0.9 3,899,879 + Shew_3261 0.54 +5.6 3,900,228 - +1.1 3,900,278 - -1.4
Or see this region's nucleotide sequence