Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3127 and Shew_3128 are separated by 338 nucleotides Shew_3128 and Shew_3129 are separated by 159 nucleotides Shew_3129 and Shew_3130 are separated by 50 nucleotides
Shew_3127: Shew_3127 - aminopeptidase B (RefSeq), at 3,718,956 to 3,720,233
_3127
Shew_3128: Shew_3128 - sugar fermentation stimulation protein (RefSeq), at 3,720,572 to 3,721,276
_3128
Shew_3129: Shew_3129 - TraR/DksA family transcriptional regulator (RefSeq), at 3,721,436 to 3,721,879
_3129
Shew_3130: Shew_3130 - glutamate--tRNA ligase (RefSeq), at 3,721,930 to 3,722,889
_3130
Position (kb)
3720
3721
3722 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3719.574 kb on - strand, within Shew_3127 at 3719.841 kb on + strand, within Shew_3127 at 3720.095 kb on - strand, within Shew_3127 at 3720.155 kb on + strand at 3720.267 kb on + strand at 3720.323 kb on - strand at 3720.389 kb on - strand at 3720.518 kb on + strand at 3720.678 kb on - strand, within Shew_3128 at 3720.952 kb on + strand, within Shew_3128 at 3720.960 kb on - strand, within Shew_3128 at 3720.976 kb on + strand, within Shew_3128 at 3721.077 kb on + strand, within Shew_3128 at 3721.218 kb on + strand at 3722.022 kb on + strand at 3722.030 kb on - strand, within Shew_3130 at 3722.156 kb on + strand, within Shew_3130
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,719,574 - Shew_3127 0.48 +0.4 3,719,841 + Shew_3127 0.69 +2.5 3,720,095 - Shew_3127 0.89 -1.8 3,720,155 + -0.6 3,720,267 + +2.1 3,720,323 - -0.7 3,720,389 - -0.4 3,720,518 + -0.4 3,720,678 - Shew_3128 0.15 +0.8 3,720,952 + Shew_3128 0.54 -0.7 3,720,960 - Shew_3128 0.55 -3.0 3,720,976 + Shew_3128 0.57 -1.3 3,721,077 + Shew_3128 0.72 -1.9 3,721,218 + +1.1 3,722,022 + +0.9 3,722,030 - Shew_3130 0.10 +2.0 3,722,156 + Shew_3130 0.24 -1.6
Or see this region's nucleotide sequence