Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2960 and Shew_2961 are separated by 81 nucleotides Shew_2961 and Shew_2962 are separated by 290 nucleotides Shew_2962 and Shew_2963 are separated by 210 nucleotides Shew_2963 and Shew_2964 are separated by 55 nucleotides
Shew_2960: Shew_2960 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 3,508,399 to 3,513,378
_2960
Shew_2961: Shew_2961 - hypothetical protein (RefSeq), at 3,513,460 to 3,513,693
_2961
Shew_2962: Shew_2962 - malate dehydrogenase (RefSeq), at 3,513,984 to 3,515,672
_2962
Shew_2963: Shew_2963 - hypothetical protein (RefSeq), at 3,515,883 to 3,516,347
_2963
Shew_2964: Shew_2964 - beta-lactamase (RefSeq), at 3,516,403 to 3,517,545
_2964
Position (kb)
3513
3514
3515
3516 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3513.232 kb on - strand at 3513.259 kb on - strand at 3513.289 kb on - strand at 3513.501 kb on + strand, within Shew_2961 at 3513.509 kb on - strand, within Shew_2961 at 3513.509 kb on - strand, within Shew_2961 at 3513.509 kb on - strand, within Shew_2961 at 3513.509 kb on - strand, within Shew_2961 at 3513.706 kb on + strand at 3514.021 kb on + strand at 3514.302 kb on - strand, within Shew_2962 at 3514.877 kb on + strand, within Shew_2962 at 3515.101 kb on - strand, within Shew_2962 at 3515.101 kb on - strand, within Shew_2962 at 3515.120 kb on - strand, within Shew_2962 at 3515.431 kb on - strand, within Shew_2962 at 3515.503 kb on - strand, within Shew_2962 at 3515.817 kb on + strand at 3516.053 kb on + strand, within Shew_2963 at 3516.181 kb on - strand, within Shew_2963 at 3516.580 kb on - strand, within Shew_2964 at 3516.604 kb on + strand, within Shew_2964 at 3516.635 kb on - strand, within Shew_2964
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,513,232 - +0.6 3,513,259 - -3.3 3,513,289 - -0.4 3,513,501 + Shew_2961 0.18 -1.4 3,513,509 - Shew_2961 0.21 +0.7 3,513,509 - Shew_2961 0.21 +0.5 3,513,509 - Shew_2961 0.21 +0.8 3,513,509 - Shew_2961 0.21 -1.5 3,513,706 + +0.2 3,514,021 + -3.0 3,514,302 - Shew_2962 0.19 -6.0 3,514,877 + Shew_2962 0.53 -1.9 3,515,101 - Shew_2962 0.66 -4.6 3,515,101 - Shew_2962 0.66 -3.5 3,515,120 - Shew_2962 0.67 -4.8 3,515,431 - Shew_2962 0.86 -1.6 3,515,503 - Shew_2962 0.90 -5.1 3,515,817 + +0.6 3,516,053 + Shew_2963 0.37 +0.5 3,516,181 - Shew_2963 0.64 -2.8 3,516,580 - Shew_2964 0.15 -0.6 3,516,604 + Shew_2964 0.18 +0.2 3,516,635 - Shew_2964 0.20 +0.8
Or see this region's nucleotide sequence