Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2818 and Shew_2819 overlap by 20 nucleotides Shew_2819 and prfC are separated by 256 nucleotides prfC and Shew_2821 are separated by 195 nucleotides
Shew_2818: Shew_2818 - TatD-related deoxyribonuclease (RefSeq), at 3,339,664 to 3,340,494
_2818
Shew_2819: Shew_2819 - hypothetical protein (RefSeq), at 3,340,475 to 3,340,765
_2819
Shew_2820: prfC - peptide chain release factor 3 (RefSeq), at 3,341,022 to 3,342,602
prfC
Shew_2821: Shew_2821 - lipoprotein NlpI (RefSeq), at 3,342,798 to 3,343,688
_2821
Position (kb)
3341
3342
3343 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 3340.149 kb on + strand, within Shew_2818 at 3340.308 kb on + strand, within Shew_2818 at 3340.334 kb on + strand, within Shew_2818 at 3340.334 kb on + strand, within Shew_2818 at 3340.503 kb on + strand at 3340.575 kb on - strand, within Shew_2819 at 3340.603 kb on + strand, within Shew_2819 at 3340.740 kb on - strand at 3340.778 kb on + strand at 3340.791 kb on + strand at 3341.537 kb on - strand, within prfC at 3341.712 kb on + strand, within prfC at 3342.248 kb on + strand, within prfC at 3342.796 kb on + strand at 3342.796 kb on + strand at 3343.221 kb on - strand, within Shew_2821 at 3343.300 kb on - strand, within Shew_2821 at 3343.487 kb on + strand, within Shew_2821 at 3343.487 kb on + strand, within Shew_2821 at 3343.495 kb on - strand, within Shew_2821 at 3343.573 kb on + strand, within Shew_2821
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,340,149 + Shew_2818 0.58 +0.9 3,340,308 + Shew_2818 0.77 +1.1 3,340,334 + Shew_2818 0.81 -0.7 3,340,334 + Shew_2818 0.81 +0.2 3,340,503 + +0.1 3,340,575 - Shew_2819 0.34 -0.6 3,340,603 + Shew_2819 0.44 +2.0 3,340,740 - +0.3 3,340,778 + +0.2 3,340,791 + -1.5 3,341,537 - prfC Shew_2820 0.33 -4.4 3,341,712 + prfC Shew_2820 0.44 -2.5 3,342,248 + prfC Shew_2820 0.78 -2.2 3,342,796 + -0.8 3,342,796 + -0.3 3,343,221 - Shew_2821 0.47 +0.0 3,343,300 - Shew_2821 0.56 -0.7 3,343,487 + Shew_2821 0.77 +1.4 3,343,487 + Shew_2821 0.77 +6.5 3,343,495 - Shew_2821 0.78 +1.3 3,343,573 + Shew_2821 0.87 -0.2
Or see this region's nucleotide sequence