Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2607 and Shew_2608 are separated by 123 nucleotides Shew_2608 and Shew_2609 are separated by 60 nucleotides Shew_2609 and Shew_2610 overlap by 4 nucleotides
Shew_2607: Shew_2607 - putative branched-chain amino acid ABC transporter (RefSeq), at 3,073,024 to 3,074,268
_2607
Shew_2608: Shew_2608 - inner-membrane translocator (RefSeq), at 3,074,392 to 3,075,465
_2608
Shew_2609: Shew_2609 - inner-membrane translocator (RefSeq), at 3,075,526 to 3,076,419
_2609
Shew_2610: Shew_2610 - AMP-dependent synthetase and ligase (RefSeq), at 3,076,416 to 3,078,383
_2610
Position (kb)
3074
3075
3076 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3073.733 kb on + strand, within Shew_2607 at 3073.754 kb on + strand, within Shew_2607 at 3073.853 kb on - strand, within Shew_2607 at 3074.176 kb on - strand at 3074.243 kb on + strand at 3074.383 kb on + strand at 3074.413 kb on - strand at 3074.413 kb on - strand at 3074.423 kb on + strand at 3074.518 kb on - strand, within Shew_2608 at 3074.545 kb on + strand, within Shew_2608 at 3074.718 kb on - strand, within Shew_2608 at 3075.007 kb on - strand, within Shew_2608 at 3075.344 kb on + strand, within Shew_2608 at 3075.421 kb on - strand at 3075.524 kb on + strand at 3075.611 kb on + strand at 3075.763 kb on + strand, within Shew_2609 at 3076.118 kb on - strand, within Shew_2609 at 3076.343 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,073,733 + Shew_2607 0.57 -1.3 3,073,754 + Shew_2607 0.59 +0.1 3,073,853 - Shew_2607 0.67 +1.5 3,074,176 - -1.1 3,074,243 + -1.5 3,074,383 + +0.0 3,074,413 - -0.5 3,074,413 - -0.0 3,074,423 + -0.0 3,074,518 - Shew_2608 0.12 -1.7 3,074,545 + Shew_2608 0.14 +0.1 3,074,718 - Shew_2608 0.30 -1.5 3,075,007 - Shew_2608 0.57 -0.9 3,075,344 + Shew_2608 0.89 +0.5 3,075,421 - +0.8 3,075,524 + -0.2 3,075,611 + +1.0 3,075,763 + Shew_2609 0.27 +0.6 3,076,118 - Shew_2609 0.66 +0.8 3,076,343 + -2.8
Or see this region's nucleotide sequence