Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2590 and Shew_2591 are separated by 9 nucleotides Shew_2591 and Shew_2592 are separated by 403 nucleotides Shew_2592 and Shew_2593 are separated by 214 nucleotides
Shew_2590: Shew_2590 - hexapaptide repeat-containing transferase (RefSeq), at 3,056,013 to 3,056,642
_2590
Shew_2591: Shew_2591 - hypothetical protein (RefSeq), at 3,056,652 to 3,056,915
_2591
Shew_2592: Shew_2592 - peptidase M28 (RefSeq), at 3,057,319 to 3,058,905
_2592
Shew_2593: Shew_2593 - acyl-CoA synthetase (RefSeq), at 3,059,120 to 3,060,844
_2593
Position (kb)
3057
3058
3059 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3056.946 kb on + strand at 3056.954 kb on - strand at 3056.988 kb on - strand at 3057.034 kb on - strand at 3057.221 kb on - strand at 3057.221 kb on - strand at 3057.760 kb on + strand, within Shew_2592 at 3057.819 kb on + strand, within Shew_2592 at 3058.692 kb on - strand, within Shew_2592 at 3058.856 kb on + strand at 3059.729 kb on - strand, within Shew_2593 at 3059.811 kb on - strand, within Shew_2593 at 3059.816 kb on + strand, within Shew_2593 at 3059.824 kb on - strand, within Shew_2593 at 3059.893 kb on + strand, within Shew_2593
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,056,946 + +0.6 3,056,954 - -3.7 3,056,988 - -0.6 3,057,034 - -1.2 3,057,221 - -2.1 3,057,221 - -0.7 3,057,760 + Shew_2592 0.28 -1.1 3,057,819 + Shew_2592 0.32 +0.2 3,058,692 - Shew_2592 0.87 +1.1 3,058,856 + -0.8 3,059,729 - Shew_2593 0.35 -0.7 3,059,811 - Shew_2593 0.40 +0.3 3,059,816 + Shew_2593 0.40 -0.3 3,059,824 - Shew_2593 0.41 +0.7 3,059,893 + Shew_2593 0.45 +0.1
Or see this region's nucleotide sequence