Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2487 and Shew_2488 are separated by 51 nucleotides Shew_2488 and ffs are separated by 279 nucleotides ffs and Shew_2489 are separated by 165 nucleotides Shew_2489 and Shew_2490 are separated by 390 nucleotides
Shew_2487: Shew_2487 - histone deacetylase superfamily protein (RefSeq), at 2,926,619 to 2,927,521
_2487
Shew_2488: Shew_2488 - hypothetical protein (RefSeq), at 2,927,573 to 2,927,851
_2488
Shew_R0086: ffs - SRP RNA; RNA component of signal recognition particle (NCBI), at 2,928,131 to 2,928,247
ffs
Shew_2489: Shew_2489 - helix-hairpin-helix repeat-containing competence protein ComEA (RefSeq), at 2,928,413 to 2,928,712
_2489
Shew_2490: Shew_2490 - anion transporter (RefSeq), at 2,929,103 to 2,930,494
_2490
Position (kb)
2928
2929 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2928.448 kb on + strand, within Shew_2489 at 2928.456 kb on - strand, within Shew_2489 at 2928.576 kb on - strand, within Shew_2489 at 2928.601 kb on + strand, within Shew_2489 at 2928.619 kb on - strand, within Shew_2489 at 2928.731 kb on + strand at 2928.733 kb on + strand at 2928.739 kb on - strand at 2928.839 kb on - strand at 2928.905 kb on + strand at 2929.076 kb on - strand at 2929.242 kb on - strand at 2929.268 kb on + strand, within Shew_2490 at 2929.316 kb on - strand, within Shew_2490 at 2929.410 kb on + strand, within Shew_2490
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,928,448 + Shew_2489 0.12 +0.3 2,928,456 - Shew_2489 0.14 +2.2 2,928,576 - Shew_2489 0.54 +1.6 2,928,601 + Shew_2489 0.63 +0.9 2,928,619 - Shew_2489 0.69 -0.6 2,928,731 + +0.0 2,928,733 + -0.6 2,928,739 - -0.0 2,928,839 - -3.1 2,928,905 + -2.3 2,929,076 - -0.1 2,929,242 - +0.6 2,929,268 + Shew_2490 0.12 -0.5 2,929,316 - Shew_2490 0.15 -0.4 2,929,410 + Shew_2490 0.22 -1.2
Or see this region's nucleotide sequence