Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2458 and Shew_2459 are separated by 243 nucleotides Shew_2459 and Shew_2460 are separated by 106 nucleotides Shew_2460 and Shew_2461 are separated by 110 nucleotides
Shew_2458: Shew_2458 - glutathione S-transferase domain-containing protein (RefSeq), at 2,890,901 to 2,891,761
_2458
Shew_2459: Shew_2459 - hypothetical protein (RefSeq), at 2,892,005 to 2,892,352
_2459
Shew_2460: Shew_2460 - hypothetical protein (RefSeq), at 2,892,459 to 2,893,502
_2460
Shew_2461: Shew_2461 - MORN repeat-containing protein (RefSeq), at 2,893,613 to 2,895,931
_2461
Position (kb)
2892
2893
2894 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2891.696 kb on + strand at 2891.728 kb on - strand at 2891.751 kb on - strand at 2891.837 kb on + strand at 2892.242 kb on + strand, within Shew_2459 at 2892.465 kb on - strand at 2892.581 kb on + strand, within Shew_2460 at 2892.582 kb on - strand, within Shew_2460 at 2892.589 kb on - strand, within Shew_2460 at 2893.229 kb on - strand, within Shew_2460 at 2893.461 kb on + strand at 2893.837 kb on + strand at 2894.040 kb on + strand, within Shew_2461 at 2894.040 kb on + strand, within Shew_2461 at 2894.048 kb on - strand, within Shew_2461 at 2894.131 kb on - strand, within Shew_2461 at 2894.293 kb on + strand, within Shew_2461 at 2894.307 kb on - strand, within Shew_2461 at 2894.448 kb on + strand, within Shew_2461
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,891,696 + +0.1 2,891,728 - -0.9 2,891,751 - +1.2 2,891,837 + -1.1 2,892,242 + Shew_2459 0.68 -0.7 2,892,465 - +0.1 2,892,581 + Shew_2460 0.12 -1.6 2,892,582 - Shew_2460 0.12 +2.0 2,892,589 - Shew_2460 0.12 +0.4 2,893,229 - Shew_2460 0.74 +1.3 2,893,461 + +0.4 2,893,837 + +1.7 2,894,040 + Shew_2461 0.18 +2.0 2,894,040 + Shew_2461 0.18 -1.6 2,894,048 - Shew_2461 0.19 +1.8 2,894,131 - Shew_2461 0.22 +0.3 2,894,293 + Shew_2461 0.29 -2.2 2,894,307 - Shew_2461 0.30 -0.9 2,894,448 + Shew_2461 0.36 -1.6
Or see this region's nucleotide sequence