Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2288 and Shew_2289 are separated by 478 nucleotides Shew_2289 and Shew_2290 are separated by 98 nucleotides Shew_2290 and Shew_2291 overlap by 1 nucleotides
Shew_2288: Shew_2288 - galactose-1-phosphate uridylyltransferase (RefSeq), at 2,673,231 to 2,674,322
_2288
Shew_2289: Shew_2289 - glycosy hydrolase family protein (RefSeq), at 2,674,801 to 2,678,010
_2289
Shew_2290: Shew_2290 - hypothetical protein (RefSeq), at 2,678,109 to 2,678,579
_2290
Shew_2291: Shew_2291 - hypothetical protein (RefSeq), at 2,678,579 to 2,679,115
_2291
Position (kb)
2674
2675
2676
2677
2678
2679 Strain fitness (log2 ratio)
-2
-1
0
1 at 2673.910 kb on - strand, within Shew_2288 at 2673.952 kb on - strand, within Shew_2288 at 2674.088 kb on - strand, within Shew_2288 at 2674.729 kb on + strand at 2674.829 kb on + strand at 2674.874 kb on - strand at 2674.911 kb on - strand at 2675.193 kb on - strand, within Shew_2289 at 2675.717 kb on + strand, within Shew_2289 at 2675.882 kb on + strand, within Shew_2289 at 2675.882 kb on + strand, within Shew_2289 at 2675.882 kb on + strand, within Shew_2289 at 2675.890 kb on - strand, within Shew_2289 at 2675.890 kb on - strand, within Shew_2289 at 2676.357 kb on + strand, within Shew_2289 at 2676.379 kb on + strand, within Shew_2289 at 2676.886 kb on + strand, within Shew_2289 at 2676.942 kb on - strand, within Shew_2289 at 2676.942 kb on - strand, within Shew_2289 at 2677.367 kb on - strand, within Shew_2289 at 2677.407 kb on + strand, within Shew_2289 at 2677.534 kb on + strand, within Shew_2289 at 2678.315 kb on + strand, within Shew_2290 at 2678.315 kb on + strand, within Shew_2290 at 2678.317 kb on - strand, within Shew_2290 at 2678.323 kb on - strand, within Shew_2290 at 2678.510 kb on - strand, within Shew_2290 at 2678.545 kb on + strand at 2678.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,673,910 - Shew_2288 0.62 -1.5 2,673,952 - Shew_2288 0.66 +0.4 2,674,088 - Shew_2288 0.78 +0.4 2,674,729 + +0.3 2,674,829 + -0.8 2,674,874 - -2.1 2,674,911 - +0.5 2,675,193 - Shew_2289 0.12 +0.1 2,675,717 + Shew_2289 0.29 -1.5 2,675,882 + Shew_2289 0.34 +1.0 2,675,882 + Shew_2289 0.34 +1.4 2,675,882 + Shew_2289 0.34 +0.8 2,675,890 - Shew_2289 0.34 +0.2 2,675,890 - Shew_2289 0.34 +0.4 2,676,357 + Shew_2289 0.48 +0.9 2,676,379 + Shew_2289 0.49 -0.1 2,676,886 + Shew_2289 0.65 +0.4 2,676,942 - Shew_2289 0.67 -2.3 2,676,942 - Shew_2289 0.67 +0.1 2,677,367 - Shew_2289 0.80 -0.3 2,677,407 + Shew_2289 0.81 +0.1 2,677,534 + Shew_2289 0.85 +0.6 2,678,315 + Shew_2290 0.44 +0.3 2,678,315 + Shew_2290 0.44 -0.3 2,678,317 - Shew_2290 0.44 -0.6 2,678,323 - Shew_2290 0.45 -0.4 2,678,510 - Shew_2290 0.85 -0.5 2,678,545 + -0.2 2,678,553 - +0.1
Or see this region's nucleotide sequence