Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2160 and Shew_2161 are separated by 263 nucleotides Shew_2161 and cpdB are separated by 305 nucleotides
Shew_2160: Shew_2160 - hypothetical protein (RefSeq), at 2,506,721 to 2,508,457
_2160
Shew_2161: Shew_2161 - peptidyl-dipeptidase Dcp (RefSeq), at 2,508,721 to 2,510,901
_2161
Shew_2162: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq), at 2,511,207 to 2,513,291
cpdB
Position (kb)
2508
2509
2510
2511 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2507.873 kb on + strand, within Shew_2160 at 2508.154 kb on + strand, within Shew_2160 at 2508.179 kb on + strand, within Shew_2160 at 2508.179 kb on + strand, within Shew_2160 at 2508.195 kb on - strand, within Shew_2160 at 2508.328 kb on + strand at 2508.417 kb on - strand at 2508.611 kb on - strand at 2508.734 kb on - strand at 2508.948 kb on - strand, within Shew_2161 at 2509.069 kb on - strand, within Shew_2161 at 2509.082 kb on + strand, within Shew_2161 at 2509.208 kb on - strand, within Shew_2161 at 2509.218 kb on - strand, within Shew_2161 at 2509.308 kb on - strand, within Shew_2161 at 2509.431 kb on + strand, within Shew_2161 at 2509.587 kb on + strand, within Shew_2161 at 2509.902 kb on - strand, within Shew_2161 at 2509.962 kb on + strand, within Shew_2161 at 2509.970 kb on - strand, within Shew_2161 at 2510.258 kb on - strand, within Shew_2161 at 2510.879 kb on + strand at 2511.101 kb on + strand at 2511.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,507,873 + Shew_2160 0.66 +2.6 2,508,154 + Shew_2160 0.82 +0.7 2,508,179 + Shew_2160 0.84 -0.5 2,508,179 + Shew_2160 0.84 +1.2 2,508,195 - Shew_2160 0.85 +0.6 2,508,328 + -3.1 2,508,417 - +0.1 2,508,611 - -0.4 2,508,734 - +0.1 2,508,948 - Shew_2161 0.10 -1.3 2,509,069 - Shew_2161 0.16 +1.1 2,509,082 + Shew_2161 0.17 +0.1 2,509,208 - Shew_2161 0.22 +1.0 2,509,218 - Shew_2161 0.23 +1.0 2,509,308 - Shew_2161 0.27 +0.8 2,509,431 + Shew_2161 0.33 -0.2 2,509,587 + Shew_2161 0.40 +0.9 2,509,902 - Shew_2161 0.54 -3.6 2,509,962 + Shew_2161 0.57 +0.6 2,509,970 - Shew_2161 0.57 +0.8 2,510,258 - Shew_2161 0.70 +0.5 2,510,879 + +0.6 2,511,101 + -0.1 2,511,109 - +1.1
Or see this region's nucleotide sequence