Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2147 and Shew_2148 are separated by 229 nucleotides Shew_2148 and Shew_2149 are separated by 86 nucleotides Shew_2149 and Shew_2150 are separated by 136 nucleotides Shew_2150 and Shew_2151 are separated by 116 nucleotides
Shew_2147: Shew_2147 - hypothetical protein (RefSeq), at 2,493,419 to 2,494,345
_2147
Shew_2148: Shew_2148 - hypothetical protein (RefSeq), at 2,494,575 to 2,494,982
_2148
Shew_2149: Shew_2149 - ABC transporter (RefSeq), at 2,495,069 to 2,495,929
_2149
Shew_2150: Shew_2150 - integral membrane protein TerC (RefSeq), at 2,496,066 to 2,496,293
_2150
Shew_2151: Shew_2151 - hypothetical protein (RefSeq), at 2,496,410 to 2,496,787
_2151
Position (kb)
2495
2496 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2494.195 kb on + strand, within Shew_2147 at 2494.311 kb on - strand at 2494.378 kb on + strand at 2494.386 kb on - strand at 2494.684 kb on - strand, within Shew_2148 at 2495.032 kb on + strand at 2495.186 kb on - strand, within Shew_2149 at 2495.668 kb on + strand, within Shew_2149 at 2495.776 kb on - strand, within Shew_2149 at 2495.881 kb on - strand at 2496.151 kb on + strand, within Shew_2150 at 2496.197 kb on - strand, within Shew_2150 at 2496.267 kb on - strand, within Shew_2150 at 2496.440 kb on + strand at 2496.440 kb on + strand at 2496.495 kb on - strand, within Shew_2151
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,494,195 + Shew_2147 0.84 +0.1 2,494,311 - +1.5 2,494,378 + -0.4 2,494,386 - -0.1 2,494,684 - Shew_2148 0.27 +0.5 2,495,032 + -0.3 2,495,186 - Shew_2149 0.14 +0.7 2,495,668 + Shew_2149 0.70 +0.9 2,495,776 - Shew_2149 0.82 -0.3 2,495,881 - -1.0 2,496,151 + Shew_2150 0.37 +1.6 2,496,197 - Shew_2150 0.57 +0.7 2,496,267 - Shew_2150 0.88 -1.4 2,496,440 + +3.7 2,496,440 + +1.5 2,496,495 - Shew_2151 0.22 -0.2
Or see this region's nucleotide sequence