Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2112 and Shew_2113 are separated by 44 nucleotides Shew_2113 and Shew_2114 are separated by 137 nucleotides Shew_2114 and Shew_2115 are separated by 95 nucleotides
Shew_2112: Shew_2112 - ribonuclease H (RefSeq), at 2,447,746 to 2,448,222
_2112
Shew_2113: Shew_2113 - hypothetical protein (RefSeq), at 2,448,267 to 2,448,989
_2113
Shew_2114: Shew_2114 - hydroxyacylglutathione hydrolase (RefSeq), at 2,449,127 to 2,449,909
_2114
Shew_2115: Shew_2115 - lytic transglycosylase, catalytic (RefSeq), at 2,450,005 to 2,451,540
_2115
Position (kb)
2449
2450 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2448.274 kb on + strand at 2448.985 kb on - strand at 2449.243 kb on - strand, within Shew_2114 at 2449.411 kb on + strand, within Shew_2114 at 2449.484 kb on - strand, within Shew_2114 at 2449.484 kb on - strand, within Shew_2114 at 2449.529 kb on - strand, within Shew_2114 at 2449.959 kb on - strand at 2450.147 kb on - strand at 2450.281 kb on - strand, within Shew_2115 at 2450.301 kb on - strand, within Shew_2115 at 2450.525 kb on - strand, within Shew_2115 at 2450.611 kb on + strand, within Shew_2115 at 2450.661 kb on - strand, within Shew_2115 at 2450.682 kb on + strand, within Shew_2115 at 2450.780 kb on - strand, within Shew_2115
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,448,274 + +0.1 2,448,985 - -1.4 2,449,243 - Shew_2114 0.15 -1.1 2,449,411 + Shew_2114 0.36 -1.1 2,449,484 - Shew_2114 0.46 -2.0 2,449,484 - Shew_2114 0.46 -2.6 2,449,529 - Shew_2114 0.51 -1.3 2,449,959 - -1.5 2,450,147 - -2.6 2,450,281 - Shew_2115 0.18 -2.6 2,450,301 - Shew_2115 0.19 +0.3 2,450,525 - Shew_2115 0.34 -0.6 2,450,611 + Shew_2115 0.39 +1.1 2,450,661 - Shew_2115 0.43 -3.3 2,450,682 + Shew_2115 0.44 +0.8 2,450,780 - Shew_2115 0.50 -1.9
Or see this region's nucleotide sequence