Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1985 and Shew_1986 are separated by 12 nucleotides Shew_1986 and Shew_1987 are separated by 15 nucleotides Shew_1987 and Shew_1988 overlap by 1 nucleotides Shew_1988 and Shew_1989 are separated by 110 nucleotides Shew_1989 and Shew_1990 are separated by 6 nucleotides
Shew_1985: Shew_1985 - cytochrome c oxidase, cbb3-type, subunit I (RefSeq), at 2,297,760 to 2,299,187
_1985
Shew_1986: Shew_1986 - cytochrome c oxidase, cbb3-type, subunit II (RefSeq), at 2,299,200 to 2,299,805
_1986
Shew_1987: Shew_1987 - Cbb3-type cytochrome oxidase component (RefSeq), at 2,299,821 to 2,299,988
_1987
Shew_1988: Shew_1988 - cytochrome c oxidase, cbb3-type, subunit III (RefSeq), at 2,299,988 to 2,300,959
_1988
Shew_1989: Shew_1989 - hypothetical protein (RefSeq), at 2,301,070 to 2,301,549
_1989
Shew_1990: Shew_1990 - heavy metal translocating P-type ATPase (RefSeq), at 2,301,556 to 2,303,979
_1990
Position (kb) 2299
2300
2301 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 2299.292 kb on - strand, within Shew_1986 at 2299.892 kb on + strand, within Shew_1987 at 2299.982 kb on - strand at 2300.241 kb on - strand, within Shew_1988 at 2301.326 kb on - strand, within Shew_1989
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,299,292 - Shew_1986 0.15 +3.5 2,299,892 + Shew_1987 0.42 +4.1 2,299,982 - +4.0 2,300,241 - Shew_1988 0.26 +4.4 2,301,326 - Shew_1989 0.53 +4.9
Or see this region's nucleotide sequence