Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1880 and Shew_1881 are separated by 192 nucleotides Shew_1881 and Shew_1882 are separated by 1 nucleotides Shew_1882 and Shew_1883 are separated by 375 nucleotides
Shew_1880: Shew_1880 - hypothetical protein (RefSeq), at 2,164,791 to 2,165,231
_1880
Shew_1881: Shew_1881 - hypothetical protein (RefSeq), at 2,165,424 to 2,165,825
_1881
Shew_1882: Shew_1882 - metal dependent phosphohydrolase (RefSeq), at 2,165,827 to 2,167,113
_1882
Shew_1883: Shew_1883 - hypothetical protein (RefSeq), at 2,167,489 to 2,167,725
_1883
Position (kb)
2165
2166
2167
2168 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2164.944 kb on - strand, within Shew_1880 at 2165.394 kb on + strand at 2165.573 kb on + strand, within Shew_1881 at 2166.006 kb on - strand, within Shew_1882 at 2166.262 kb on - strand, within Shew_1882 at 2166.446 kb on + strand, within Shew_1882 at 2167.401 kb on - strand at 2167.664 kb on + strand, within Shew_1883 at 2167.708 kb on + strand at 2167.967 kb on - strand at 2168.097 kb on - strand at 2168.111 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,164,944 - Shew_1880 0.35 +0.9 2,165,394 + +0.4 2,165,573 + Shew_1881 0.37 -0.7 2,166,006 - Shew_1882 0.14 -2.0 2,166,262 - Shew_1882 0.34 +1.0 2,166,446 + Shew_1882 0.48 +0.5 2,167,401 - +1.7 2,167,664 + Shew_1883 0.74 -4.0 2,167,708 + -0.5 2,167,967 - -2.0 2,168,097 - +2.2 2,168,111 + -2.0
Or see this region's nucleotide sequence