Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1803 and Shew_1804 are separated by 144 nucleotides Shew_1804 and Shew_1805 are separated by 325 nucleotides Shew_1805 and Shew_1806 are separated by 18 nucleotides
Shew_1803: Shew_1803 - aminopeptidase N (RefSeq), at 2,064,418 to 2,066,979
_1803
Shew_1804: Shew_1804 - hypothetical protein (RefSeq), at 2,067,124 to 2,067,378
_1804
Shew_1805: Shew_1805 - hypothetical protein (RefSeq), at 2,067,704 to 2,069,752
_1805
Shew_1806: Shew_1806 - hypothetical protein (RefSeq), at 2,069,771 to 2,071,135
_1806
Position (kb)
2067
2068
2069
2070 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2067.035 kb on - strand at 2067.112 kb on - strand at 2067.256 kb on + strand, within Shew_1804 at 2067.769 kb on - strand at 2067.776 kb on - strand at 2068.044 kb on + strand, within Shew_1805 at 2068.054 kb on + strand, within Shew_1805 at 2068.183 kb on + strand, within Shew_1805 at 2068.353 kb on + strand, within Shew_1805 at 2068.353 kb on + strand, within Shew_1805 at 2068.353 kb on + strand, within Shew_1805 at 2068.780 kb on + strand, within Shew_1805 at 2069.218 kb on + strand, within Shew_1805 at 2069.443 kb on + strand, within Shew_1805 at 2069.600 kb on + strand at 2069.916 kb on + strand, within Shew_1806 at 2070.538 kb on - strand, within Shew_1806 at 2070.544 kb on + strand, within Shew_1806 at 2070.610 kb on + strand, within Shew_1806 at 2070.654 kb on - strand, within Shew_1806 at 2070.752 kb on - strand, within Shew_1806
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,067,035 - -2.7 2,067,112 - -0.4 2,067,256 + Shew_1804 0.52 -2.4 2,067,769 - +0.4 2,067,776 - -0.1 2,068,044 + Shew_1805 0.17 -0.5 2,068,054 + Shew_1805 0.17 +0.8 2,068,183 + Shew_1805 0.23 +0.8 2,068,353 + Shew_1805 0.32 -0.5 2,068,353 + Shew_1805 0.32 +1.5 2,068,353 + Shew_1805 0.32 +1.8 2,068,780 + Shew_1805 0.53 +1.7 2,069,218 + Shew_1805 0.74 +1.2 2,069,443 + Shew_1805 0.85 -2.2 2,069,600 + -0.7 2,069,916 + Shew_1806 0.11 -1.5 2,070,538 - Shew_1806 0.56 -2.6 2,070,544 + Shew_1806 0.57 -1.4 2,070,610 + Shew_1806 0.61 -0.8 2,070,654 - Shew_1806 0.65 -1.4 2,070,752 - Shew_1806 0.72 +1.1
Or see this region's nucleotide sequence