Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1731 and Shew_1732 are separated by 30 nucleotides Shew_1732 and Shew_1733 overlap by 4 nucleotides Shew_1733 and Shew_1734 are separated by 30 nucleotides Shew_1734 and Shew_1735 are separated by 321 nucleotides
Shew_1731: Shew_1731 - histidine triad (HIT) protein (RefSeq), at 1,983,289 to 1,983,639
_1731
Shew_1732: Shew_1732 - hypothetical protein (RefSeq), at 1,983,670 to 1,984,167
_1732
Shew_1733: Shew_1733 - thioesterase superfamily protein (RefSeq), at 1,984,164 to 1,984,628
_1733
Shew_1734: Shew_1734 - hypothetical protein (RefSeq), at 1,984,659 to 1,985,048
_1734
Shew_1735: Shew_1735 - TonB-dependent receptor (RefSeq), at 1,985,370 to 1,987,511
_1735
Position (kb) 1984
1985 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1983.389 kb on - strand, within Shew_1731 at 1983.660 kb on + strand at 1983.668 kb on - strand at 1983.806 kb on + strand, within Shew_1732 at 1983.991 kb on + strand, within Shew_1732 at 1984.120 kb on - strand at 1984.120 kb on - strand at 1984.449 kb on - strand, within Shew_1733 at 1984.945 kb on + strand, within Shew_1734 at 1984.945 kb on + strand, within Shew_1734
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,983,389 - Shew_1731 0.28 -2.6 1,983,660 + +0.1 1,983,668 - +0.2 1,983,806 + Shew_1732 0.27 -0.1 1,983,991 + Shew_1732 0.64 -3.1 1,984,120 - +1.5 1,984,120 - -2.9 1,984,449 - Shew_1733 0.61 -1.9 1,984,945 + Shew_1734 0.73 -2.1 1,984,945 + Shew_1734 0.73 +1.5
Or see this region's nucleotide sequence