Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1563 and Shew_1564 are separated by 125 nucleotides Shew_1564 and Shew_1565 are separated by 244 nucleotides Shew_1565 and Shew_1566 are separated by 35 nucleotides
Shew_1563: Shew_1563 - isocitrate dehydrogenase, NADP-dependent (RefSeq), at 1,793,391 to 1,795,610
_1563
Shew_1564: Shew_1564 - cold-shock DNA-binding domain-containing protein (RefSeq), at 1,795,736 to 1,795,942
_1564
Shew_1565: Shew_1565 - ATP-dependent Clp protease adaptor protein ClpS (RefSeq), at 1,796,187 to 1,796,495
_1565
Shew_1566: Shew_1566 - ATP-dependent Clp protease, ATP-binding subunit clpA (RefSeq), at 1,796,531 to 1,798,789
_1566
Position (kb) 1796
1797 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 1795.415 kb on + strand at 1795.496 kb on + strand at 1795.783 kb on + strand, within Shew_1564 at 1795.807 kb on + strand, within Shew_1564 at 1796.125 kb on + strand at 1796.133 kb on - strand at 1796.466 kb on + strand at 1796.474 kb on + strand at 1797.443 kb on - strand, within Shew_1566 at 1797.480 kb on + strand, within Shew_1566
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,795,415 + +7.9 1,795,496 + +8.3 1,795,783 + Shew_1564 0.23 -5.0 1,795,807 + Shew_1564 0.34 -1.1 1,796,125 + -2.6 1,796,133 - -1.6 1,796,466 + -2.4 1,796,474 + -4.1 1,797,443 - Shew_1566 0.40 -0.4 1,797,480 + Shew_1566 0.42 +3.3
Or see this region's nucleotide sequence