Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1506 and Shew_1507 are separated by 2 nucleotides Shew_1507 and Shew_1508 are separated by 73 nucleotides Shew_1508 and asnB are separated by 75 nucleotides
Shew_1506: Shew_1506 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 1,728,569 to 1,728,919
_1506
Shew_1507: Shew_1507 - 5-carboxymethyl-2-hydroxymuconate isomerase (RefSeq), at 1,728,922 to 1,729,269
_1507
Shew_1508: Shew_1508 - extracellular solute-binding protein (RefSeq), at 1,729,343 to 1,730,116
_1508
Shew_1509: asnB - asparagine synthetase B (RefSeq), at 1,730,192 to 1,731,850
asnB
Position (kb)
1729
1730
1731 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1728.578 kb on + strand at 1728.581 kb on - strand at 1729.587 kb on + strand, within Shew_1508 at 1729.595 kb on - strand, within Shew_1508 at 1729.595 kb on - strand, within Shew_1508 at 1729.605 kb on - strand, within Shew_1508 at 1730.202 kb on - strand at 1730.403 kb on + strand, within asnB at 1730.667 kb on - strand, within asnB at 1730.935 kb on + strand, within asnB at 1731.037 kb on - strand, within asnB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,728,578 + -0.4 1,728,581 - -1.1 1,729,587 + Shew_1508 0.32 +0.2 1,729,595 - Shew_1508 0.33 -0.6 1,729,595 - Shew_1508 0.33 -0.3 1,729,605 - Shew_1508 0.34 -1.1 1,730,202 - -1.2 1,730,403 + asnB Shew_1509 0.13 -0.0 1,730,667 - asnB Shew_1509 0.29 -3.7 1,730,935 + asnB Shew_1509 0.45 -6.4 1,731,037 - asnB Shew_1509 0.51 -1.6
Or see this region's nucleotide sequence