Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1382 and fliA overlap by 8 nucleotides fliA and Shew_1384 are separated by 42 nucleotides Shew_1384 and Shew_1385 are separated by 13 nucleotides Shew_1385 and Shew_1386 are separated by 15 nucleotides
Shew_1382: Shew_1382 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,591,829 to 1,592,740
_1382
Shew_1383: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,592,733 to 1,593,452
fliA
Shew_1384: Shew_1384 - response regulator receiver protein (RefSeq), at 1,593,495 to 1,593,878
_1384
Shew_1385: Shew_1385 - chemotaxis phosphatase, CheZ (RefSeq), at 1,593,892 to 1,594,629
_1385
Shew_1386: Shew_1386 - CheA signal transduction histidine kinase (RefSeq), at 1,594,645 to 1,596,885
_1386
Position (kb)
1593
1594 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1592.872 kb on - strand, within fliA at 1592.874 kb on + strand, within fliA at 1593.035 kb on - strand, within fliA at 1593.695 kb on + strand, within Shew_1384 at 1593.905 kb on + strand at 1593.930 kb on + strand at 1594.241 kb on - strand, within Shew_1385 at 1594.277 kb on + strand, within Shew_1385 at 1594.323 kb on - strand, within Shew_1385 at 1594.410 kb on - strand, within Shew_1385 at 1594.450 kb on + strand, within Shew_1385 at 1594.772 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,592,872 - fliA Shew_1383 0.19 +0.9 1,592,874 + fliA Shew_1383 0.20 -3.7 1,593,035 - fliA Shew_1383 0.42 +0.9 1,593,695 + Shew_1384 0.52 -2.5 1,593,905 + +1.1 1,593,930 + -2.3 1,594,241 - Shew_1385 0.47 +1.9 1,594,277 + Shew_1385 0.52 +0.3 1,594,323 - Shew_1385 0.58 +1.4 1,594,410 - Shew_1385 0.70 +0.8 1,594,450 + Shew_1385 0.76 +0.7 1,594,772 - +2.2
Or see this region's nucleotide sequence