Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1263 and Shew_1264 overlap by 4 nucleotides Shew_1264 and Shew_1265 are separated by 72 nucleotides Shew_1265 and Shew_1266 are separated by 4 nucleotides
Shew_1263: Shew_1263 - hypothetical protein (RefSeq), at 1,461,001 to 1,461,984
_1263
Shew_1264: Shew_1264 - UvrD/REP helicase (RefSeq), at 1,461,981 to 1,464,017
_1264
Shew_1265: Shew_1265 - hypothetical protein (RefSeq), at 1,464,090 to 1,464,632
_1265
Shew_1266: Shew_1266 - XRE family transcriptional regulator (RefSeq), at 1,464,637 to 1,465,020
_1266
Position (kb)
1461
1462
1463
1464
1465 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1461.019 kb on + strand at 1461.066 kb on - strand at 1461.076 kb on - strand at 1461.577 kb on + strand, within Shew_1263 at 1461.579 kb on + strand, within Shew_1263 at 1461.663 kb on - strand, within Shew_1263 at 1461.844 kb on - strand, within Shew_1263 at 1461.925 kb on + strand at 1462.061 kb on - strand at 1462.082 kb on + strand at 1462.191 kb on + strand, within Shew_1264 at 1463.166 kb on - strand, within Shew_1264 at 1464.012 kb on - strand at 1464.307 kb on - strand, within Shew_1265 at 1464.825 kb on + strand, within Shew_1266
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,461,019 + +1.1 1,461,066 - -0.1 1,461,076 - +0.4 1,461,577 + Shew_1263 0.59 +0.5 1,461,579 + Shew_1263 0.59 -0.6 1,461,663 - Shew_1263 0.67 -0.0 1,461,844 - Shew_1263 0.86 -0.2 1,461,925 + +0.1 1,462,061 - -2.2 1,462,082 + +1.3 1,462,191 + Shew_1264 0.10 -2.2 1,463,166 - Shew_1264 0.58 -3.2 1,464,012 - -1.5 1,464,307 - Shew_1265 0.40 +0.9 1,464,825 + Shew_1266 0.49 -0.8
Or see this region's nucleotide sequence