Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1252 and Shew_1253 are separated by 89 nucleotides Shew_1253 and Shew_1254 are separated by 72 nucleotides Shew_1254 and Shew_1255 are separated by 109 nucleotides Shew_1255 and Shew_1256 are separated by 79 nucleotides
Shew_1252: Shew_1252 - transposase (RefSeq), at 1,450,808 to 1,451,473
_1252
Shew_1253: Shew_1253 - retinol acyltransferase domain-containing protein (RefSeq), at 1,451,563 to 1,452,288
_1253
Shew_1254: Shew_1254 - phage integrase family protein (RefSeq), at 1,452,361 to 1,452,921
_1254
Shew_1255: Shew_1255 - hypothetical protein (RefSeq), at 1,453,031 to 1,453,435
_1255
Shew_1256: Shew_1256 - hypothetical protein (RefSeq), at 1,453,515 to 1,453,877
_1256
Position (kb)
1452
1453 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1451.416 kb on + strand at 1451.416 kb on + strand at 1451.476 kb on + strand at 1451.532 kb on + strand at 1451.540 kb on - strand at 1451.559 kb on - strand at 1451.683 kb on - strand, within Shew_1253 at 1451.806 kb on + strand, within Shew_1253 at 1451.811 kb on + strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.819 kb on - strand, within Shew_1253 at 1451.922 kb on + strand, within Shew_1253 at 1451.931 kb on + strand, within Shew_1253 at 1452.127 kb on - strand, within Shew_1253 at 1452.228 kb on + strand at 1452.228 kb on + strand at 1452.228 kb on + strand at 1452.310 kb on + strand at 1452.320 kb on + strand at 1452.333 kb on + strand at 1452.346 kb on - strand at 1452.536 kb on + strand, within Shew_1254 at 1452.794 kb on + strand, within Shew_1254 at 1452.804 kb on + strand, within Shew_1254 at 1452.804 kb on + strand, within Shew_1254 at 1453.488 kb on - strand at 1453.622 kb on + strand, within Shew_1256 at 1453.630 kb on - strand, within Shew_1256 at 1453.697 kb on - strand, within Shew_1256 at 1453.708 kb on + strand, within Shew_1256 at 1453.708 kb on + strand, within Shew_1256 at 1453.716 kb on - strand, within Shew_1256 at 1453.781 kb on + strand, within Shew_1256 at 1453.795 kb on - strand, within Shew_1256 at 1453.833 kb on - strand, within Shew_1256 at 1453.833 kb on - strand, within Shew_1256
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,451,416 + -1.1 1,451,416 + -1.9 1,451,476 + +0.1 1,451,532 + -2.2 1,451,540 - -0.3 1,451,559 - -0.9 1,451,683 - Shew_1253 0.17 -1.9 1,451,806 + Shew_1253 0.33 +0.3 1,451,811 + Shew_1253 0.34 +0.7 1,451,814 - Shew_1253 0.35 -0.4 1,451,814 - Shew_1253 0.35 +0.1 1,451,814 - Shew_1253 0.35 -0.6 1,451,819 - Shew_1253 0.35 -1.8 1,451,922 + Shew_1253 0.49 -2.5 1,451,931 + Shew_1253 0.51 -3.2 1,452,127 - Shew_1253 0.78 +0.2 1,452,228 + +0.6 1,452,228 + +0.5 1,452,228 + +1.3 1,452,310 + -1.5 1,452,320 + -2.5 1,452,333 + -0.1 1,452,346 - +0.5 1,452,536 + Shew_1254 0.31 +1.2 1,452,794 + Shew_1254 0.77 +0.2 1,452,804 + Shew_1254 0.79 +0.1 1,452,804 + Shew_1254 0.79 +2.4 1,453,488 - -2.5 1,453,622 + Shew_1256 0.29 -0.2 1,453,630 - Shew_1256 0.32 -2.2 1,453,697 - Shew_1256 0.50 +1.8 1,453,708 + Shew_1256 0.53 +1.5 1,453,708 + Shew_1256 0.53 +0.4 1,453,716 - Shew_1256 0.55 -0.7 1,453,781 + Shew_1256 0.73 -3.4 1,453,795 - Shew_1256 0.77 +0.7 1,453,833 - Shew_1256 0.88 -0.0 1,453,833 - Shew_1256 0.88 +0.7
Or see this region's nucleotide sequence