Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1143 and Shew_1144 are separated by 21 nucleotides Shew_1144 and Shew_1145 are separated by 16 nucleotides Shew_1145 and Shew_1146 are separated by 216 nucleotides
Shew_1143: Shew_1143 - Holliday junction resolvase-like protein (RefSeq), at 1,320,077 to 1,320,496
_1143
Shew_1144: Shew_1144 - hypothetical protein (RefSeq), at 1,320,518 to 1,321,075
_1144
Shew_1145: Shew_1145 - translation initiation factor Sui1 (RefSeq), at 1,321,092 to 1,321,421
_1145
Shew_1146: Shew_1146 - hypothetical protein (RefSeq), at 1,321,638 to 1,321,952
_1146
Position (kb)
1320
1321
1322 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1319.742 kb on - strand at 1319.742 kb on - strand at 1319.855 kb on + strand at 1319.858 kb on - strand at 1320.688 kb on - strand, within Shew_1144 at 1320.923 kb on - strand, within Shew_1144 at 1321.138 kb on + strand, within Shew_1145 at 1321.146 kb on - strand, within Shew_1145 at 1321.146 kb on - strand, within Shew_1145 at 1321.184 kb on + strand, within Shew_1145 at 1321.345 kb on + strand, within Shew_1145 at 1321.695 kb on - strand, within Shew_1146 at 1321.874 kb on - strand, within Shew_1146 at 1322.060 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,319,742 - +0.1 1,319,742 - +1.8 1,319,855 + +2.6 1,319,858 - +0.6 1,320,688 - Shew_1144 0.30 -0.2 1,320,923 - Shew_1144 0.73 -0.2 1,321,138 + Shew_1145 0.14 +4.8 1,321,146 - Shew_1145 0.16 -3.3 1,321,146 - Shew_1145 0.16 -3.7 1,321,184 + Shew_1145 0.28 +5.2 1,321,345 + Shew_1145 0.77 +5.4 1,321,695 - Shew_1146 0.18 +1.5 1,321,874 - Shew_1146 0.75 -0.0 1,322,060 + -0.2
Or see this region's nucleotide sequence