Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1132 and Shew_1133 are separated by 69 nucleotides Shew_1133 and Shew_1134 are separated by 86 nucleotides Shew_1134 and Shew_1135 are separated by 0 nucleotides Shew_1135 and Shew_1136 are separated by 60 nucleotides
Shew_1132: Shew_1132 - RdgB/HAM1 family non-canonical purine NTP pyrophosphatase (RefSeq), at 1,308,471 to 1,309,142
_1132
Shew_1133: Shew_1133 - hypothetical protein (RefSeq), at 1,309,212 to 1,309,646
_1133
Shew_1134: Shew_1134 - hypothetical protein (RefSeq), at 1,309,733 to 1,310,023
_1134
Shew_1135: Shew_1135 - protein of unknown function YGGT (RefSeq), at 1,310,024 to 1,310,572
_1135
Shew_1136: Shew_1136 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 1,310,633 to 1,311,451
_1136
Position (kb)
1309
1310
1311 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1309.196 kb on + strand at 1309.249 kb on + strand at 1309.367 kb on + strand, within Shew_1133 at 1309.375 kb on - strand, within Shew_1133 at 1309.536 kb on - strand, within Shew_1133 at 1309.546 kb on - strand, within Shew_1133 at 1309.701 kb on - strand at 1309.739 kb on + strand at 1309.747 kb on - strand at 1309.850 kb on - strand, within Shew_1134 at 1309.918 kb on + strand, within Shew_1134 at 1309.918 kb on + strand, within Shew_1134 at 1309.918 kb on + strand, within Shew_1134 at 1309.970 kb on - strand, within Shew_1134 at 1310.666 kb on - strand at 1310.700 kb on - strand at 1310.849 kb on + strand, within Shew_1136
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,309,196 + -1.7 1,309,249 + +0.3 1,309,367 + Shew_1133 0.36 +0.3 1,309,375 - Shew_1133 0.37 +0.1 1,309,536 - Shew_1133 0.74 +0.1 1,309,546 - Shew_1133 0.77 -0.7 1,309,701 - +1.9 1,309,739 + -0.9 1,309,747 - +1.1 1,309,850 - Shew_1134 0.40 -0.2 1,309,918 + Shew_1134 0.64 +0.8 1,309,918 + Shew_1134 0.64 +0.3 1,309,918 + Shew_1134 0.64 -3.3 1,309,970 - Shew_1134 0.81 -1.3 1,310,666 - -5.6 1,310,700 - -4.7 1,310,849 + Shew_1136 0.26 -6.7
Or see this region's nucleotide sequence