Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1123 and trmB are separated by 235 nucleotides trmB and Shew_1125 are separated by 30 nucleotides Shew_1125 and Shew_1126 overlap by 1 nucleotides
Shew_1123: Shew_1123 - A/G-specific adenine glycosylase (RefSeq), at 1,298,053 to 1,299,159
_1123
Shew_1124: trmB - tRNA (guanine-N(7))-methyltransferase (RefSeq), at 1,299,395 to 1,300,111
trmB
Shew_1125: Shew_1125 - hypothetical protein (RefSeq), at 1,300,142 to 1,300,465
_1125
Shew_1126: Shew_1126 - glutaminase (RefSeq), at 1,300,465 to 1,301,379
_1126
Position (kb) 1300
1301 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1299.419 kb on - strand at 1299.464 kb on - strand at 1299.740 kb on - strand, within trmB at 1299.801 kb on + strand, within trmB at 1300.247 kb on - strand, within Shew_1125 at 1301.179 kb on + strand, within Shew_1126 at 1301.179 kb on - strand, within Shew_1126 at 1301.184 kb on + strand, within Shew_1126 at 1301.356 kb on + strand at 1301.356 kb on + strand at 1301.356 kb on + strand at 1301.359 kb on - strand at 1301.428 kb on + strand at 1301.446 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,299,419 - -3.4 1,299,464 - -4.3 1,299,740 - trmB Shew_1124 0.48 -3.2 1,299,801 + trmB Shew_1124 0.57 -3.5 1,300,247 - Shew_1125 0.32 -3.8 1,301,179 + Shew_1126 0.78 -1.7 1,301,179 - Shew_1126 0.78 -1.7 1,301,184 + Shew_1126 0.79 -2.3 1,301,356 + -6.5 1,301,356 + -4.1 1,301,356 + -3.9 1,301,359 - -4.2 1,301,428 + +0.5 1,301,446 - -2.0
Or see this region's nucleotide sequence