Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0729 and Shew_0730 are separated by 88 nucleotides Shew_0730 and Shew_0731 are separated by 8 nucleotides Shew_0731 and Shew_0732 are separated by 282 nucleotides
Shew_0729: Shew_0729 - FAD-dependent pyridine nucleotide-disulphide oxidoreductase (RefSeq), at 830,300 to 832,000
_0729
Shew_0730: Shew_0730 - protein of unknown function DUF395, YeeE/YedE (RefSeq), at 832,089 to 832,517
_0730
Shew_0731: Shew_0731 - protein of unknown function DUF395, YeeE/YedE (RefSeq), at 832,526 to 832,996
_0731
Shew_0732: Shew_0732 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 833,279 to 834,619
_0732
Position (kb) 832
833 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 831.494 kb on - strand, within Shew_0729 at 831.644 kb on + strand, within Shew_0729 at 831.652 kb on - strand, within Shew_0729 at 832.113 kb on - strand at 832.123 kb on - strand at 832.467 kb on + strand, within Shew_0730 at 832.636 kb on + strand, within Shew_0731 at 832.724 kb on - strand, within Shew_0731 at 832.825 kb on - strand, within Shew_0731 at 833.142 kb on + strand at 833.469 kb on - strand, within Shew_0732 at 833.485 kb on + strand, within Shew_0732 at 833.485 kb on + strand, within Shew_0732
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 831,494 - Shew_0729 0.70 +0.2 831,644 + Shew_0729 0.79 +2.6 831,652 - Shew_0729 0.79 +0.6 832,113 - -0.2 832,123 - -0.3 832,467 + Shew_0730 0.88 -2.9 832,636 + Shew_0731 0.23 -0.6 832,724 - Shew_0731 0.42 -0.2 832,825 - Shew_0731 0.63 -1.5 833,142 + -1.0 833,469 - Shew_0732 0.14 -2.6 833,485 + Shew_0732 0.15 -0.0 833,485 + Shew_0732 0.15 -0.3
Or see this region's nucleotide sequence