Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0713 and cobT are separated by 122 nucleotides cobT and Shew_0715 are separated by 41 nucleotides Shew_0715 and Shew_0716 overlap by 4 nucleotides
Shew_0713: Shew_0713 - transport system permease protein (RefSeq), at 813,329 to 814,372
_0713
Shew_0714: cobT - nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (RefSeq), at 814,495 to 815,523
cobT
Shew_0715: Shew_0715 - cobalamin 5'-phosphate synthase (RefSeq), at 815,565 to 816,332
_0715
Shew_0716: Shew_0716 - cobalbumin biosynthesis enzyme (RefSeq), at 816,329 to 816,877
_0716
Position (kb) 814
815
816 Strain fitness (log2 ratio) -2
-1
0
1 at 813.657 kb on + strand, within Shew_0713 at 813.726 kb on + strand, within Shew_0713 at 813.891 kb on - strand, within Shew_0713 at 813.909 kb on + strand, within Shew_0713 at 813.914 kb on + strand, within Shew_0713 at 813.917 kb on - strand, within Shew_0713 at 813.927 kb on - strand, within Shew_0713 at 814.056 kb on + strand, within Shew_0713 at 814.781 kb on - strand, within cobT at 814.879 kb on + strand, within cobT at 815.578 kb on + strand at 815.720 kb on + strand, within Shew_0715 at 815.728 kb on - strand, within Shew_0715 at 815.944 kb on - strand, within Shew_0715 at 816.002 kb on - strand, within Shew_0715 at 816.079 kb on - strand, within Shew_0715 at 816.304 kb on - strand at 816.411 kb on + strand, within Shew_0716 at 816.485 kb on + strand, within Shew_0716 at 816.518 kb on + strand, within Shew_0716
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 813,657 + Shew_0713 0.31 +0.2 813,726 + Shew_0713 0.38 -2.6 813,891 - Shew_0713 0.54 -0.4 813,909 + Shew_0713 0.56 -2.6 813,914 + Shew_0713 0.56 +0.8 813,917 - Shew_0713 0.56 -0.4 813,927 - Shew_0713 0.57 -2.3 814,056 + Shew_0713 0.70 -1.3 814,781 - cobT Shew_0714 0.28 -0.2 814,879 + cobT Shew_0714 0.37 -1.2 815,578 + +0.5 815,720 + Shew_0715 0.20 +0.2 815,728 - Shew_0715 0.21 -1.6 815,944 - Shew_0715 0.49 -2.0 816,002 - Shew_0715 0.57 -1.0 816,079 - Shew_0715 0.67 -1.0 816,304 - -1.0 816,411 + Shew_0716 0.15 +1.2 816,485 + Shew_0716 0.28 +1.0 816,518 + Shew_0716 0.34 -0.4
Or see this region's nucleotide sequence