Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0563 and Shew_0564 are separated by 9 nucleotides Shew_0564 and Shew_0565 overlap by 8 nucleotides
Shew_0563: Shew_0563 - N-acetylmuramoyl-L-alanine amidase (RefSeq), at 637,936 to 639,255
_0563
Shew_0564: Shew_0564 - DNA mismatch repair protein MutL (RefSeq), at 639,265 to 641,160
_0564
Shew_0565: Shew_0565 - tRNA delta(2)-isopentenylpyrophosphate transferase (RefSeq), at 641,153 to 642,079
_0565
Position (kb) 639
640
641
642 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 638.948 kb on + strand, within Shew_0563 at 639.209 kb on - strand at 639.355 kb on + strand at 639.363 kb on - strand at 639.363 kb on - strand at 639.478 kb on - strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.613 kb on - strand, within Shew_0564 at 639.638 kb on - strand, within Shew_0564 at 639.652 kb on - strand, within Shew_0564 at 639.962 kb on + strand, within Shew_0564 at 640.178 kb on + strand, within Shew_0564 at 640.186 kb on - strand, within Shew_0564 at 640.225 kb on - strand, within Shew_0564 at 640.519 kb on + strand, within Shew_0564 at 640.586 kb on - strand, within Shew_0564 at 640.643 kb on + strand, within Shew_0564 at 640.699 kb on + strand, within Shew_0564 at 641.242 kb on + strand at 641.590 kb on + strand, within Shew_0565 at 641.717 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 638,948 + Shew_0563 0.77 -0.9 639,209 - +4.7 639,355 + +2.1 639,363 - +1.2 639,363 - +1.7 639,478 - Shew_0564 0.11 +1.6 639,494 + Shew_0564 0.12 +1.9 639,494 + Shew_0564 0.12 +1.1 639,494 + Shew_0564 0.12 +1.7 639,613 - Shew_0564 0.18 +2.1 639,638 - Shew_0564 0.20 +2.6 639,652 - Shew_0564 0.20 +0.5 639,962 + Shew_0564 0.37 +1.3 640,178 + Shew_0564 0.48 +2.0 640,186 - Shew_0564 0.49 -0.1 640,225 - Shew_0564 0.51 -2.0 640,519 + Shew_0564 0.66 +2.8 640,586 - Shew_0564 0.70 -0.7 640,643 + Shew_0564 0.73 +1.7 640,699 + Shew_0564 0.76 +1.6 641,242 + -2.8 641,590 + Shew_0565 0.47 -0.4 641,717 + Shew_0565 0.61 -2.2 641,768 + Shew_0565 0.66 -1.0 641,768 + Shew_0565 0.66 -1.7 641,987 + -0.4
Or see this region's nucleotide sequence