Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0019 and Shew_R0020 are separated by 45 nucleotides Shew_R0020 and Shew_R0021 are separated by 7 nucleotides Shew_R0021 and Shew_R0022 are separated by 8 nucleotides Shew_R0022 and Shew_0560 are separated by 700 nucleotides Shew_0560 and Shew_0561 are separated by 436 nucleotides
Shew_R0019: Shew_R0019 - tRNA-Gly (RefSeq), at 633,941 to 634,016
_R0019
Shew_R0020: Shew_R0020 - tRNA-Gly (RefSeq), at 634,062 to 634,137
_R0020
Shew_R0021: Shew_R0021 - tRNA-Gly (RefSeq), at 634,145 to 634,220
_R0021
Shew_R0022: Shew_R0022 - tRNA-Gly (RefSeq), at 634,229 to 634,304
_R0022
Shew_0560: Shew_0560 - hypothetical protein (RefSeq), at 635,005 to 635,460
_0560
Shew_0561: Shew_0561 - carbohydrate kinase, YjeF-related protein (RefSeq), at 635,897 to 637,375
_0561
Position (kb) 635
636 Strain fitness (log2 ratio) -1
0
1 at 634.535 kb on + strand at 634.891 kb on + strand at 635.031 kb on + strand at 635.049 kb on - strand at 635.088 kb on - strand, within Shew_0560 at 635.090 kb on + strand, within Shew_0560 at 635.090 kb on + strand, within Shew_0560 at 635.098 kb on - strand, within Shew_0560 at 635.362 kb on - strand, within Shew_0560 at 635.454 kb on + strand at 635.462 kb on - strand at 635.772 kb on + strand at 635.854 kb on + strand at 635.899 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 634,535 + -1.4 634,891 + +1.4 635,031 + +0.3 635,049 - -0.4 635,088 - Shew_0560 0.18 -1.4 635,090 + Shew_0560 0.19 +1.1 635,090 + Shew_0560 0.19 +1.2 635,098 - Shew_0560 0.20 +1.6 635,362 - Shew_0560 0.78 +1.3 635,454 + +0.0 635,462 - -1.2 635,772 + +1.4 635,854 + -0.2 635,899 - +0.3
Or see this region's nucleotide sequence