Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0533 and Shew_0534 are separated by 202 nucleotides Shew_0534 and Shew_0535 are separated by 72 nucleotides Shew_0535 and Shew_0536 are separated by 195 nucleotides
Shew_0533: Shew_0533 - excinuclease ABC, A subunit (RefSeq), at 603,761 to 606,586
_0533
Shew_0534: Shew_0534 - major facilitator transporter (RefSeq), at 606,789 to 608,156
_0534
Shew_0535: Shew_0535 - single-strand binding protein (RefSeq), at 608,229 to 608,891
_0535
Shew_0536: Shew_0536 - tRNA-dihydrouridine synthase A (RefSeq), at 609,087 to 610,076
_0536
Position (kb)
606
607
608
609 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 605.962 kb on + strand, within Shew_0533 at 606.076 kb on - strand, within Shew_0533 at 606.094 kb on - strand, within Shew_0533 at 606.370 kb on + strand at 606.442 kb on - strand at 606.466 kb on - strand at 606.493 kb on + strand at 606.571 kb on + strand at 606.593 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.814 kb on - strand at 606.814 kb on - strand at 607.367 kb on - strand, within Shew_0534 at 607.597 kb on - strand, within Shew_0534 at 607.615 kb on + strand, within Shew_0534 at 607.783 kb on - strand, within Shew_0534 at 607.952 kb on + strand, within Shew_0534 at 608.962 kb on - strand at 608.972 kb on - strand at 608.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 605,962 + Shew_0533 0.78 -0.7 606,076 - Shew_0533 0.82 +0.4 606,094 - Shew_0533 0.83 -1.3 606,370 + +0.2 606,442 - -0.5 606,466 - -1.8 606,493 + +1.3 606,571 + +0.5 606,593 + -2.1 606,806 + +4.6 606,806 + +3.7 606,806 + +4.3 606,806 + +4.2 606,806 + +2.8 606,806 + +3.6 606,814 - +4.9 606,814 - +6.5 607,367 - Shew_0534 0.42 +4.1 607,597 - Shew_0534 0.59 +4.5 607,615 + Shew_0534 0.60 +3.6 607,783 - Shew_0534 0.73 +4.5 607,952 + Shew_0534 0.85 +0.8 608,962 - -0.3 608,972 - -0.6 608,990 + -0.3
Or see this region's nucleotide sequence