Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metL and metF are separated by 263 nucleotides metF and Shew_0523 are separated by 124 nucleotides Shew_0523 and Shew_0524 are separated by 10 nucleotides
Shew_0521: metL - bifunctional aspartate kinase II/homoserine dehydrogenase II (RefSeq), at 590,362 to 592,755
metL
Shew_0522: metF - 5,10-methylenetetrahydrofolate reductase (RefSeq), at 593,019 to 593,909
metF
Shew_0523: Shew_0523 - cytochrome c family protein (RefSeq), at 594,034 to 594,633
_0523
Shew_0524: Shew_0524 - cytochrome c, class I (RefSeq), at 594,644 to 595,705
_0524
Position (kb) 593
594 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 592.082 kb on - strand, within metL at 592.169 kb on + strand, within metL at 592.169 kb on + strand, within metL at 592.177 kb on - strand, within metL at 592.204 kb on - strand, within metL at 592.540 kb on - strand at 592.548 kb on + strand at 593.299 kb on - strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.392 kb on + strand, within metF at 593.400 kb on - strand, within metF at 593.400 kb on - strand, within metF at 593.400 kb on - strand, within metF at 593.400 kb on - strand, within metF at 593.446 kb on - strand, within metF at 593.746 kb on + strand, within metF at 593.844 kb on + strand at 593.844 kb on + strand at 593.845 kb on - strand at 593.852 kb on - strand at 593.886 kb on - strand at 594.027 kb on + strand at 594.128 kb on - strand, within Shew_0523 at 594.337 kb on - strand, within Shew_0523 at 594.400 kb on - strand, within Shew_0523 at 594.465 kb on + strand, within Shew_0523 at 594.895 kb on - strand, within Shew_0524
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 592,082 - metL Shew_0521 0.72 -1.6 592,169 + metL Shew_0521 0.75 +0.0 592,169 + metL Shew_0521 0.75 -0.5 592,177 - metL Shew_0521 0.76 +0.0 592,204 - metL Shew_0521 0.77 +0.4 592,540 - +1.1 592,548 + -0.5 593,299 - metF Shew_0522 0.31 -7.2 593,392 + metF Shew_0522 0.42 -4.6 593,392 + metF Shew_0522 0.42 -3.5 593,392 + metF Shew_0522 0.42 -7.2 593,392 + metF Shew_0522 0.42 -5.0 593,392 + metF Shew_0522 0.42 -7.0 593,392 + metF Shew_0522 0.42 -5.4 593,392 + metF Shew_0522 0.42 -6.1 593,400 - metF Shew_0522 0.43 -6.0 593,400 - metF Shew_0522 0.43 -3.0 593,400 - metF Shew_0522 0.43 -3.9 593,400 - metF Shew_0522 0.43 -0.3 593,446 - metF Shew_0522 0.48 -2.7 593,746 + metF Shew_0522 0.82 -3.1 593,844 + -6.1 593,844 + -6.7 593,845 - -4.5 593,852 - -7.3 593,886 - -2.5 594,027 + +1.2 594,128 - Shew_0523 0.16 +0.5 594,337 - Shew_0523 0.51 -0.6 594,400 - Shew_0523 0.61 -0.9 594,465 + Shew_0523 0.72 -0.3 594,895 - Shew_0524 0.24 +1.0
Or see this region's nucleotide sequence