Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0459 and Shew_0460 are separated by 222 nucleotides Shew_0460 and Shew_0461 are separated by 72 nucleotides Shew_0461 and Shew_0462 are separated by 312 nucleotides
Shew_0459: Shew_0459 - PAS/PAC sensor hybrid histidine kinase (RefSeq), at 512,269 to 515,961
_0459
Shew_0460: Shew_0460 - hypothetical protein (RefSeq), at 516,184 to 518,583
_0460
Shew_0461: Shew_0461 - hypothetical protein (RefSeq), at 518,656 to 519,195
_0461
Shew_0462: Shew_0462 - hypothetical protein (RefSeq), at 519,508 to 519,813
_0462
Position (kb) 516
517
518
519 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 515.815 kb on - strand at 516.098 kb on - strand at 516.588 kb on + strand, within Shew_0460 at 516.667 kb on - strand, within Shew_0460 at 516.680 kb on + strand, within Shew_0460 at 516.961 kb on + strand, within Shew_0460 at 517.023 kb on - strand, within Shew_0460 at 517.094 kb on - strand, within Shew_0460 at 517.506 kb on + strand, within Shew_0460 at 517.506 kb on + strand, within Shew_0460 at 517.935 kb on - strand, within Shew_0460 at 518.174 kb on + strand, within Shew_0460 at 518.339 kb on - strand, within Shew_0460 at 518.791 kb on - strand, within Shew_0461 at 518.833 kb on + strand, within Shew_0461 at 519.049 kb on + strand, within Shew_0461 at 519.157 kb on - strand at 519.454 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 515,815 - +0.7 516,098 - +1.7 516,588 + Shew_0460 0.17 -0.9 516,667 - Shew_0460 0.20 +0.4 516,680 + Shew_0460 0.21 -0.7 516,961 + Shew_0460 0.32 -1.9 517,023 - Shew_0460 0.35 +1.0 517,094 - Shew_0460 0.38 +1.4 517,506 + Shew_0460 0.55 +2.2 517,506 + Shew_0460 0.55 -0.3 517,935 - Shew_0460 0.73 -0.3 518,174 + Shew_0460 0.83 -0.0 518,339 - Shew_0460 0.90 +0.0 518,791 - Shew_0461 0.25 -0.4 518,833 + Shew_0461 0.33 +0.6 519,049 + Shew_0461 0.73 +0.3 519,157 - -1.3 519,454 + -3.0
Or see this region's nucleotide sequence