Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0413 and Shew_0414 overlap by 4 nucleotides Shew_0414 and Shew_0415 are separated by 36 nucleotides Shew_0415 and Shew_0416 are separated by 146 nucleotides
Shew_0413: Shew_0413 - ABC-2 type transporter (RefSeq), at 466,858 to 468,030
_0413
Shew_0414: Shew_0414 - ABC-2 type transporter (RefSeq), at 468,027 to 469,172
_0414
Shew_0415: Shew_0415 - hypothetical protein (RefSeq), at 469,209 to 469,751
_0415
Shew_0416: Shew_0416 - peptidase U62, modulator of DNA gyrase (RefSeq), at 469,898 to 471,238
_0416
Position (kb)
468
469
470 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 467.115 kb on + strand, within Shew_0413 at 467.381 kb on - strand, within Shew_0413 at 467.438 kb on - strand, within Shew_0413 at 467.438 kb on - strand, within Shew_0413 at 467.542 kb on + strand, within Shew_0413 at 467.686 kb on + strand, within Shew_0413 at 467.994 kb on - strand at 468.064 kb on - strand at 468.193 kb on + strand, within Shew_0414 at 468.303 kb on - strand, within Shew_0414 at 468.426 kb on + strand, within Shew_0414 at 468.427 kb on + strand, within Shew_0414 at 468.620 kb on - strand, within Shew_0414 at 468.721 kb on + strand, within Shew_0414 at 468.786 kb on - strand, within Shew_0414 at 468.852 kb on - strand, within Shew_0414 at 469.364 kb on - strand, within Shew_0415 at 469.515 kb on - strand, within Shew_0415 at 469.560 kb on - strand, within Shew_0415 at 469.715 kb on + strand at 469.724 kb on + strand at 469.732 kb on - strand at 470.142 kb on - strand, within Shew_0416
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 467,115 + Shew_0413 0.22 -0.4 467,381 - Shew_0413 0.45 +2.5 467,438 - Shew_0413 0.49 +0.3 467,438 - Shew_0413 0.49 -0.1 467,542 + Shew_0413 0.58 -0.1 467,686 + Shew_0413 0.71 +2.2 467,994 - -0.2 468,064 - +0.8 468,193 + Shew_0414 0.14 -0.0 468,303 - Shew_0414 0.24 +0.9 468,426 + Shew_0414 0.35 -2.0 468,427 + Shew_0414 0.35 -1.2 468,620 - Shew_0414 0.52 -0.8 468,721 + Shew_0414 0.61 -0.2 468,786 - Shew_0414 0.66 -0.0 468,852 - Shew_0414 0.72 +3.3 469,364 - Shew_0415 0.29 -0.8 469,515 - Shew_0415 0.56 -3.3 469,560 - Shew_0415 0.65 -1.0 469,715 + +2.4 469,724 + +2.4 469,732 - -2.3 470,142 - Shew_0416 0.18 +0.9
Or see this region's nucleotide sequence