Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0411 and Shew_0412 overlap by 11 nucleotides Shew_0412 and Shew_0413 are separated by 12 nucleotides Shew_0413 and Shew_0414 overlap by 4 nucleotides
Shew_0411: Shew_0411 - outer membrane efflux protein (RefSeq), at 464,481 to 465,884
_0411
Shew_0412: Shew_0412 - secretion protein HlyD family protein (RefSeq), at 465,874 to 466,845
_0412
Shew_0413: Shew_0413 - ABC-2 type transporter (RefSeq), at 466,858 to 468,030
_0413
Shew_0414: Shew_0414 - ABC-2 type transporter (RefSeq), at 468,027 to 469,172
_0414
Position (kb) 466
467
468
469 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 465.858 kb on + strand at 466.097 kb on + strand, within Shew_0412 at 466.499 kb on + strand, within Shew_0412 at 466.519 kb on + strand, within Shew_0412 at 466.606 kb on - strand, within Shew_0412 at 466.636 kb on - strand, within Shew_0412 at 466.827 kb on - strand at 466.861 kb on + strand at 466.983 kb on - strand, within Shew_0413 at 467.115 kb on + strand, within Shew_0413 at 467.381 kb on - strand, within Shew_0413 at 467.438 kb on - strand, within Shew_0413 at 467.438 kb on - strand, within Shew_0413 at 467.542 kb on + strand, within Shew_0413 at 467.686 kb on + strand, within Shew_0413 at 467.994 kb on - strand at 468.064 kb on - strand at 468.193 kb on + strand, within Shew_0414 at 468.303 kb on - strand, within Shew_0414 at 468.426 kb on + strand, within Shew_0414 at 468.427 kb on + strand, within Shew_0414 at 468.620 kb on - strand, within Shew_0414 at 468.721 kb on + strand, within Shew_0414 at 468.786 kb on - strand, within Shew_0414 at 468.852 kb on - strand, within Shew_0414
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 465,858 + +0.3 466,097 + Shew_0412 0.23 -0.8 466,499 + Shew_0412 0.64 +0.7 466,519 + Shew_0412 0.66 -3.7 466,606 - Shew_0412 0.75 -1.5 466,636 - Shew_0412 0.78 -0.2 466,827 - -0.0 466,861 + -3.3 466,983 - Shew_0413 0.11 +1.8 467,115 + Shew_0413 0.22 -0.4 467,381 - Shew_0413 0.45 +2.5 467,438 - Shew_0413 0.49 +0.3 467,438 - Shew_0413 0.49 -0.1 467,542 + Shew_0413 0.58 -0.1 467,686 + Shew_0413 0.71 +2.2 467,994 - -0.2 468,064 - +0.8 468,193 + Shew_0414 0.14 -0.0 468,303 - Shew_0414 0.24 +0.9 468,426 + Shew_0414 0.35 -2.0 468,427 + Shew_0414 0.35 -1.2 468,620 - Shew_0414 0.52 -0.8 468,721 + Shew_0414 0.61 -0.2 468,786 - Shew_0414 0.66 -0.0 468,852 - Shew_0414 0.72 +3.3
Or see this region's nucleotide sequence