Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0400 and Shew_0401 overlap by 11 nucleotides Shew_0401 and Shew_0402 overlap by 8 nucleotides Shew_0402 and Shew_0403 are separated by 165 nucleotides
Shew_0400: Shew_0400 - methylation site containing protein (RefSeq), at 448,909 to 449,706
_0400
Shew_0401: Shew_0401 - MSHA biogenesis protein MshP (RefSeq), at 449,696 to 450,133
_0401
Shew_0402: Shew_0402 - MSHA biogenesis protein MshQ (RefSeq), at 450,126 to 452,828
_0402
Shew_0403: Shew_0403 - cell shape determining protein MreB (RefSeq), at 452,994 to 454,043
_0403
Position (kb)
450
451
452
453 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 449.252 kb on + strand, within Shew_0400 at 449.313 kb on - strand, within Shew_0400 at 449.363 kb on - strand, within Shew_0400 at 449.418 kb on - strand, within Shew_0400 at 449.418 kb on - strand, within Shew_0400 at 449.642 kb on + strand at 449.989 kb on - strand, within Shew_0401 at 450.014 kb on + strand, within Shew_0401 at 450.170 kb on + strand at 450.321 kb on - strand at 451.130 kb on + strand, within Shew_0402 at 451.583 kb on + strand, within Shew_0402 at 451.583 kb on + strand, within Shew_0402 at 451.591 kb on - strand, within Shew_0402 at 451.643 kb on + strand, within Shew_0402 at 451.784 kb on - strand, within Shew_0402 at 451.814 kb on + strand, within Shew_0402 at 451.814 kb on + strand, within Shew_0402 at 451.852 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 452.080 kb on + strand, within Shew_0402 at 452.088 kb on - strand, within Shew_0402 at 452.536 kb on + strand, within Shew_0402 at 452.549 kb on - strand, within Shew_0402 at 452.680 kb on + strand at 452.747 kb on - strand at 452.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 449,252 + Shew_0400 0.43 -3.1 449,313 - Shew_0400 0.51 -0.6 449,363 - Shew_0400 0.57 -3.0 449,418 - Shew_0400 0.64 -3.8 449,418 - Shew_0400 0.64 -3.7 449,642 + -2.5 449,989 - Shew_0401 0.67 -1.9 450,014 + Shew_0401 0.73 -2.2 450,170 + -3.3 450,321 - -1.2 451,130 + Shew_0402 0.37 -3.5 451,583 + Shew_0402 0.54 -1.6 451,583 + Shew_0402 0.54 -2.9 451,591 - Shew_0402 0.54 -2.5 451,643 + Shew_0402 0.56 -5.2 451,784 - Shew_0402 0.61 -3.7 451,814 + Shew_0402 0.62 -1.2 451,814 + Shew_0402 0.62 -3.5 451,852 + Shew_0402 0.64 -2.6 451,884 + Shew_0402 0.65 -0.2 451,884 + Shew_0402 0.65 -2.5 451,884 + Shew_0402 0.65 -4.4 451,884 + Shew_0402 0.65 -3.4 451,884 + Shew_0402 0.65 -2.2 452,080 + Shew_0402 0.72 -2.3 452,088 - Shew_0402 0.73 -2.2 452,536 + Shew_0402 0.89 -3.2 452,549 - Shew_0402 0.90 -3.6 452,680 + -2.3 452,747 - -2.5 452,859 - +0.2
Or see this region's nucleotide sequence