Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0384 and Shew_0385 are separated by 236 nucleotides Shew_0385 and Shew_0386 are separated by 0 nucleotides Shew_0386 and Shew_0387 overlap by 4 nucleotides
Shew_0384: Shew_0384 - regulatory protein CsrD (RefSeq), at 434,107 to 436,017
_0384
Shew_0385: Shew_0385 - biogenesis protein MshI (RefSeq), at 436,254 to 437,132
_0385
Shew_0386: Shew_0386 - fimbrial assembly family protein (RefSeq), at 437,133 to 437,738
_0386
Shew_0387: Shew_0387 - MSHA biogenesis protein MshJ (RefSeq), at 437,735 to 438,391
_0387
Position (kb)
436
437
438 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 435.398 kb on + strand, within Shew_0384 at 435.398 kb on + strand, within Shew_0384 at 435.444 kb on + strand, within Shew_0384 at 435.452 kb on - strand, within Shew_0384 at 435.565 kb on - strand, within Shew_0384 at 435.792 kb on - strand, within Shew_0384 at 435.971 kb on + strand at 435.979 kb on - strand at 436.052 kb on + strand at 436.664 kb on + strand, within Shew_0385 at 436.664 kb on + strand, within Shew_0385 at 436.886 kb on - strand, within Shew_0385 at 437.362 kb on - strand, within Shew_0386 at 437.536 kb on + strand, within Shew_0386 at 437.643 kb on + strand, within Shew_0386 at 437.874 kb on + strand, within Shew_0387 at 437.986 kb on + strand, within Shew_0387
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 435,398 + Shew_0384 0.68 -2.2 435,398 + Shew_0384 0.68 -3.0 435,444 + Shew_0384 0.70 -0.8 435,452 - Shew_0384 0.70 -0.3 435,565 - Shew_0384 0.76 -1.4 435,792 - Shew_0384 0.88 +0.7 435,971 + -0.9 435,979 - -3.0 436,052 + +1.6 436,664 + Shew_0385 0.47 -2.8 436,664 + Shew_0385 0.47 -3.7 436,886 - Shew_0385 0.72 -3.7 437,362 - Shew_0386 0.38 -1.9 437,536 + Shew_0386 0.67 -4.1 437,643 + Shew_0386 0.84 -3.1 437,874 + Shew_0387 0.21 -2.3 437,986 + Shew_0387 0.38 -2.8
Or see this region's nucleotide sequence