Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0380 and rpmE are separated by 180 nucleotides rpmE and Shew_0382 are separated by 226 nucleotides Shew_0382 and Shew_0383 are separated by 179 nucleotides
Shew_0380: Shew_0380 - hypothetical protein (RefSeq), at 430,339 to 431,115
_0380
Shew_0381: rpmE - 50S ribosomal protein L31 (RefSeq), at 431,296 to 431,508
rpmE
Shew_0382: Shew_0382 - nuclease (RefSeq), at 431,735 to 432,505
_0382
Shew_0383: Shew_0383 - malate dehydrogenase (RefSeq), at 432,685 to 433,932
_0383
Position (kb) 431
432
433 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 430.797 kb on - strand, within Shew_0380 at 431.181 kb on - strand at 431.625 kb on - strand at 431.625 kb on - strand at 431.644 kb on + strand at 431.765 kb on - strand at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 432.098 kb on - strand, within Shew_0382 at 432.583 kb on + strand at 432.591 kb on - strand at 432.792 kb on + strand at 433.246 kb on + strand, within Shew_0383 at 433.254 kb on - strand, within Shew_0383 at 433.498 kb on + strand, within Shew_0383
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 430,797 - Shew_0380 0.59 -3.9 431,181 - +0.5 431,625 - +1.3 431,625 - +0.2 431,644 + -0.6 431,765 - +0.9 431,928 + Shew_0382 0.25 -1.4 431,928 + Shew_0382 0.25 -0.2 431,928 + Shew_0382 0.25 +1.0 431,928 + Shew_0382 0.25 +1.0 431,928 + Shew_0382 0.25 -0.3 431,928 + Shew_0382 0.25 -0.0 431,936 - Shew_0382 0.26 -1.0 431,936 - Shew_0382 0.26 +0.4 431,936 - Shew_0382 0.26 -0.6 431,936 - Shew_0382 0.26 -1.2 431,936 - Shew_0382 0.26 -0.3 431,936 - Shew_0382 0.26 +0.1 431,936 - Shew_0382 0.26 -0.0 432,098 - Shew_0382 0.47 -0.5 432,583 + +1.7 432,591 - +0.8 432,792 + +1.0 433,246 + Shew_0383 0.45 +1.0 433,254 - Shew_0383 0.46 +1.5 433,498 + Shew_0383 0.65 +1.7
Or see this region's nucleotide sequence