Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0362 and Shew_0363 are separated by 117 nucleotides Shew_0363 and Shew_0364 are separated by 11 nucleotides Shew_0364 and Shew_R0018 are separated by 189 nucleotides Shew_R0018 and Shew_0365 are separated by 499 nucleotides
Shew_0362: Shew_0362 - signal peptidase I (RefSeq), at 410,865 to 411,581
_0362
Shew_0363: Shew_0363 - nucleotidase (RefSeq), at 411,699 to 412,376
_0363
Shew_0364: Shew_0364 - MshA, mannose-sensitive haemaglutinin (RefSeq), at 412,388 to 412,879
_0364
Shew_R0018: Shew_R0018 - tRNA-Ser (RefSeq), at 413,069 to 413,159
_R0018
Shew_0365: Shew_0365 - hypothetical protein (RefSeq), at 413,659 to 414,147
_0365
Position (kb) 412
413 Strain fitness (log2 ratio) -1
0
1
2
3 at 411.424 kb on - strand, within Shew_0362 at 411.654 kb on + strand at 411.678 kb on + strand at 411.680 kb on - strand at 412.255 kb on - strand, within Shew_0363 at 412.434 kb on - strand at 412.434 kb on - strand at 412.441 kb on - strand, within Shew_0364 at 412.609 kb on - strand, within Shew_0364 at 413.464 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 411,424 - Shew_0362 0.78 +0.3 411,654 + -0.8 411,678 + -0.6 411,680 - +0.8 412,255 - Shew_0363 0.82 +0.9 412,434 - -0.0 412,434 - -0.1 412,441 - Shew_0364 0.11 -1.2 412,609 - Shew_0364 0.45 -1.4 413,464 - +3.0
Or see this region's nucleotide sequence