Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0332 and Shew_0333 are separated by 144 nucleotides Shew_0333 and Shew_0334 are separated by 46 nucleotides Shew_0334 and sdhB overlap by 4 nucleotides sdhB and Shew_0336 are separated by 191 nucleotides
Shew_0332: Shew_0332 - fumarate reductase cytochrome b-556 subunit (RefSeq), at 377,079 to 377,801
_0332
Shew_0333: Shew_0333 - fumarate reductase respiratory complex, transmembrane subunit (RefSeq), at 377,946 to 378,617
_0333
Shew_0334: Shew_0334 - fumarate reductase flavoprotein subunit (RefSeq), at 378,664 to 380,670
_0334
Shew_0335: sdhB - fumarate reductase iron-sulfur subunit (RefSeq), at 380,667 to 381,398
sdhB
Shew_0336: Shew_0336 - LysR family transcriptional regulator (RefSeq), at 381,590 to 382,513
_0336
Position (kb) 378
379
380
381 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 377.812 kb on - strand at 378.023 kb on - strand, within Shew_0333 at 378.037 kb on - strand, within Shew_0333 at 378.047 kb on - strand, within Shew_0333 at 378.047 kb on - strand, within Shew_0333 at 378.416 kb on + strand, within Shew_0333 at 378.576 kb on + strand at 378.576 kb on + strand at 378.771 kb on + strand at 378.828 kb on + strand at 378.836 kb on - strand at 379.118 kb on - strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.151 kb on - strand, within Shew_0334 at 379.421 kb on - strand, within Shew_0334 at 379.670 kb on + strand, within Shew_0334 at 379.679 kb on + strand, within Shew_0334 at 380.125 kb on - strand, within Shew_0334 at 380.125 kb on - strand, within Shew_0334 at 380.125 kb on - strand, within Shew_0334 at 380.157 kb on + strand, within Shew_0334 at 380.163 kb on - strand, within Shew_0334 at 380.252 kb on - strand, within Shew_0334 at 380.363 kb on + strand, within Shew_0334 at 380.367 kb on - strand, within Shew_0334 at 380.371 kb on - strand, within Shew_0334 at 380.371 kb on - strand, within Shew_0334 at 380.567 kb on + strand at 380.607 kb on + strand at 380.678 kb on - strand at 380.915 kb on - strand, within sdhB at 380.999 kb on + strand, within sdhB at 381.010 kb on + strand, within sdhB at 381.155 kb on - strand, within sdhB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 377,812 - -1.4 378,023 - Shew_0333 0.11 -0.1 378,037 - Shew_0333 0.14 -2.2 378,047 - Shew_0333 0.15 -0.5 378,047 - Shew_0333 0.15 -0.9 378,416 + Shew_0333 0.70 -1.2 378,576 + -1.6 378,576 + -0.2 378,771 + +0.0 378,828 + +0.1 378,836 - -0.5 379,118 - Shew_0334 0.23 +2.3 379,143 + Shew_0334 0.24 +1.0 379,143 + Shew_0334 0.24 -3.1 379,143 + Shew_0334 0.24 +1.7 379,143 + Shew_0334 0.24 +0.6 379,143 + Shew_0334 0.24 +0.2 379,151 - Shew_0334 0.24 -2.2 379,421 - Shew_0334 0.38 +0.7 379,670 + Shew_0334 0.50 -0.2 379,679 + Shew_0334 0.51 -0.4 380,125 - Shew_0334 0.73 +0.3 380,125 - Shew_0334 0.73 +1.4 380,125 - Shew_0334 0.73 +0.3 380,157 + Shew_0334 0.74 +1.1 380,163 - Shew_0334 0.75 -1.5 380,252 - Shew_0334 0.79 -2.2 380,363 + Shew_0334 0.85 +0.2 380,367 - Shew_0334 0.85 +1.1 380,371 - Shew_0334 0.85 +0.2 380,371 - Shew_0334 0.85 -2.9 380,567 + +0.5 380,607 + -2.0 380,678 - +0.4 380,915 - sdhB Shew_0335 0.34 +0.9 380,999 + sdhB Shew_0335 0.45 +0.9 381,010 + sdhB Shew_0335 0.47 +1.8 381,155 - sdhB Shew_0335 0.67 -2.0
Or see this region's nucleotide sequence