Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0320 and Shew_0321 are separated by 489 nucleotides Shew_0321 and Shew_0322 are separated by 5 nucleotides Shew_0322 and dapF are separated by 37 nucleotides dapF and Shew_0324 are separated by 8 nucleotides
Shew_0320: Shew_0320 - frataxin family protein (RefSeq), at 367,230 to 367,604
_0320
Shew_0321: Shew_0321 - hypothetical protein (RefSeq), at 368,094 to 368,261
_0321
Shew_0322: Shew_0322 - diaminopimelate decarboxylase (RefSeq), at 368,267 to 369,511
_0322
Shew_0323: dapF - diaminopimelate epimerase (RefSeq), at 369,549 to 370,388
dapF
Shew_0324: Shew_0324 - hypothetical protein (RefSeq), at 370,397 to 371,023
_0324
Position (kb) 368
369
370 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 367.270 kb on - strand, within Shew_0320 at 367.270 kb on - strand, within Shew_0320 at 367.270 kb on - strand, within Shew_0320 at 367.313 kb on - strand, within Shew_0320 at 367.413 kb on - strand, within Shew_0320 at 368.416 kb on - strand, within Shew_0322 at 368.423 kb on + strand, within Shew_0322 at 368.449 kb on - strand, within Shew_0322 at 368.451 kb on + strand, within Shew_0322 at 368.459 kb on - strand, within Shew_0322 at 368.459 kb on - strand, within Shew_0322 at 368.723 kb on + strand, within Shew_0322 at 369.262 kb on - strand, within Shew_0322 at 369.403 kb on - strand at 369.505 kb on + strand at 369.505 kb on + strand at 370.489 kb on + strand, within Shew_0324
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 367,270 - Shew_0320 0.11 -3.3 367,270 - Shew_0320 0.11 -2.6 367,270 - Shew_0320 0.11 -2.0 367,313 - Shew_0320 0.22 -5.2 367,413 - Shew_0320 0.49 -3.5 368,416 - Shew_0322 0.12 -2.9 368,423 + Shew_0322 0.13 -3.7 368,449 - Shew_0322 0.15 -6.2 368,451 + Shew_0322 0.15 -5.9 368,459 - Shew_0322 0.15 -1.2 368,459 - Shew_0322 0.15 -0.8 368,723 + Shew_0322 0.37 -5.0 369,262 - Shew_0322 0.80 -0.2 369,403 - -5.1 369,505 + +1.1 369,505 + -0.9 370,489 + Shew_0324 0.15 -1.3
Or see this region's nucleotide sequence