Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0308 and Shew_0309 are separated by 16 nucleotides Shew_0309 and Shew_0310 overlap by 1 nucleotides Shew_0310 and Shew_0311 are separated by 31 nucleotides
Shew_0308: Shew_0308 - hypothetical protein (RefSeq), at 334,532 to 335,239
_0308
Shew_0309: Shew_0309 - OmpA/MotB domain-containing protein (RefSeq), at 335,256 to 335,861
_0309
Shew_0310: Shew_0310 - TolC family type I secretion outer membrane protein (RefSeq), at 335,861 to 337,273
_0310
Shew_0311: Shew_0311 - HlyD family type I secretion membrane fusion protein (RefSeq), at 337,305 to 338,687
_0311
Position (kb) 335
336
337
338 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 334.911 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.974 kb on + strand, within Shew_0308 at 335.049 kb on + strand, within Shew_0308 at 335.058 kb on - strand, within Shew_0308 at 335.173 kb on - strand at 335.173 kb on - strand at 335.394 kb on + strand, within Shew_0309 at 335.585 kb on + strand, within Shew_0309 at 335.873 kb on + strand at 335.894 kb on + strand at 335.954 kb on - strand at 336.260 kb on + strand, within Shew_0310 at 336.268 kb on - strand, within Shew_0310 at 336.414 kb on + strand, within Shew_0310 at 336.651 kb on - strand, within Shew_0310 at 336.711 kb on - strand, within Shew_0310 at 336.804 kb on + strand, within Shew_0310 at 337.000 kb on - strand, within Shew_0310 at 337.000 kb on - strand, within Shew_0310 at 337.017 kb on + strand, within Shew_0310 at 337.182 kb on - strand at 337.462 kb on + strand, within Shew_0311 at 337.470 kb on - strand, within Shew_0311 at 337.524 kb on - strand, within Shew_0311 at 337.891 kb on + strand, within Shew_0311 at 337.901 kb on + strand, within Shew_0311 at 338.093 kb on - strand, within Shew_0311 at 338.192 kb on + strand, within Shew_0311 at 338.251 kb on + strand, within Shew_0311 at 338.251 kb on + strand, within Shew_0311
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 334,911 - Shew_0308 0.54 +2.8 334,940 - Shew_0308 0.58 +3.3 334,940 - Shew_0308 0.58 +3.2 334,974 + Shew_0308 0.62 +2.1 335,049 + Shew_0308 0.73 -3.5 335,058 - Shew_0308 0.74 +3.1 335,173 - +3.8 335,173 - +2.9 335,394 + Shew_0309 0.23 +1.4 335,585 + Shew_0309 0.54 -3.7 335,873 + +1.7 335,894 + -1.5 335,954 - -1.5 336,260 + Shew_0310 0.28 -1.5 336,268 - Shew_0310 0.29 -1.3 336,414 + Shew_0310 0.39 -2.6 336,651 - Shew_0310 0.56 -2.6 336,711 - Shew_0310 0.60 -2.1 336,804 + Shew_0310 0.67 -1.4 337,000 - Shew_0310 0.81 -1.0 337,000 - Shew_0310 0.81 -2.0 337,017 + Shew_0310 0.82 -0.8 337,182 - -2.2 337,462 + Shew_0311 0.11 -3.1 337,470 - Shew_0311 0.12 -2.3 337,524 - Shew_0311 0.16 -2.5 337,891 + Shew_0311 0.42 -2.7 337,901 + Shew_0311 0.43 -1.6 338,093 - Shew_0311 0.57 -5.0 338,192 + Shew_0311 0.64 -1.0 338,251 + Shew_0311 0.68 -1.4 338,251 + Shew_0311 0.68 -1.3
Or see this region's nucleotide sequence