Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0285 and Shew_0286 are separated by 197 nucleotides Shew_0286 and Shew_0287 are separated by 67 nucleotides
Shew_0285: Shew_0285 - diguanylate cyclase (RefSeq), at 303,520 to 304,401
_0285
Shew_0286: Shew_0286 - extracellular solute-binding protein (RefSeq), at 304,599 to 305,408
_0286
Shew_0287: Shew_0287 - DNA-binding transcriptional regulator IlvY (RefSeq), at 305,476 to 306,360
_0287
Position (kb) 304
305
306 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 303.784 kb on - strand, within Shew_0285 at 304.174 kb on + strand, within Shew_0285 at 304.786 kb on + strand, within Shew_0286 at 304.794 kb on - strand, within Shew_0286 at 305.014 kb on - strand, within Shew_0286 at 305.014 kb on - strand, within Shew_0286 at 305.373 kb on + strand at 305.651 kb on + strand, within Shew_0287 at 305.656 kb on + strand, within Shew_0287 at 305.657 kb on - strand, within Shew_0287 at 305.709 kb on + strand, within Shew_0287 at 305.824 kb on + strand, within Shew_0287 at 306.262 kb on + strand, within Shew_0287 at 306.267 kb on + strand, within Shew_0287 at 306.270 kb on - strand, within Shew_0287 at 306.275 kb on - strand at 306.275 kb on - strand at 306.310 kb on + strand at 306.317 kb on + strand at 306.319 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 303,784 - Shew_0285 0.30 -1.4 304,174 + Shew_0285 0.74 -0.1 304,786 + Shew_0286 0.23 -0.8 304,794 - Shew_0286 0.24 +0.9 305,014 - Shew_0286 0.51 -0.5 305,014 - Shew_0286 0.51 +0.1 305,373 + -2.5 305,651 + Shew_0287 0.20 -4.2 305,656 + Shew_0287 0.20 -2.9 305,657 - Shew_0287 0.20 -3.9 305,709 + Shew_0287 0.26 -1.4 305,824 + Shew_0287 0.39 -3.7 306,262 + Shew_0287 0.89 -2.7 306,267 + Shew_0287 0.89 -0.9 306,270 - Shew_0287 0.90 -2.9 306,275 - -4.7 306,275 - -3.4 306,310 + -1.4 306,317 + -4.0 306,319 - -4.0
Or see this region's nucleotide sequence