Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0245 and Shew_0246 are separated by 153 nucleotides Shew_0246 and Shew_0247 are separated by 306 nucleotides Shew_0247 and Shew_0248 are separated by 44 nucleotides
Shew_0245: Shew_0245 - molydopterin dinucleotide-binding region (RefSeq), at 262,483 to 265,521
_0245
Shew_0246: Shew_0246 - hypothetical protein (RefSeq), at 265,675 to 266,292
_0246
Shew_0247: Shew_0247 - hypothetical protein (RefSeq), at 266,599 to 266,994
_0247
Shew_0248: Shew_0248 - GCN5-related N-acetyltransferase (RefSeq), at 267,039 to 267,596
_0248
Position (kb) 265
266
267 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 264.821 kb on + strand, within Shew_0245 at 264.829 kb on - strand, within Shew_0245 at 264.852 kb on + strand, within Shew_0245 at 264.885 kb on - strand, within Shew_0245 at 264.938 kb on - strand, within Shew_0245 at 264.938 kb on - strand, within Shew_0245 at 264.938 kb on - strand, within Shew_0245 at 265.248 kb on + strand at 265.273 kb on + strand at 265.592 kb on - strand at 265.755 kb on - strand, within Shew_0246 at 265.988 kb on + strand, within Shew_0246 at 266.568 kb on + strand at 266.576 kb on - strand at 266.616 kb on + strand at 266.688 kb on + strand, within Shew_0247 at 266.885 kb on + strand, within Shew_0247 at 266.893 kb on - strand, within Shew_0247 at 266.893 kb on - strand, within Shew_0247 at 267.192 kb on - strand, within Shew_0248 at 267.285 kb on - strand, within Shew_0248 at 267.287 kb on + strand, within Shew_0248
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 264,821 + Shew_0245 0.77 -0.4 264,829 - Shew_0245 0.77 +1.8 264,852 + Shew_0245 0.78 -0.6 264,885 - Shew_0245 0.79 +0.4 264,938 - Shew_0245 0.81 -1.0 264,938 - Shew_0245 0.81 -0.5 264,938 - Shew_0245 0.81 +1.1 265,248 + +0.8 265,273 + -2.2 265,592 - +1.6 265,755 - Shew_0246 0.13 +0.2 265,988 + Shew_0246 0.51 +0.9 266,568 + -1.2 266,576 - -2.1 266,616 + +1.0 266,688 + Shew_0247 0.22 +0.6 266,885 + Shew_0247 0.72 -3.2 266,893 - Shew_0247 0.74 -1.3 266,893 - Shew_0247 0.74 +0.7 267,192 - Shew_0248 0.27 -0.1 267,285 - Shew_0248 0.44 +1.3 267,287 + Shew_0248 0.44 +1.8
Or see this region's nucleotide sequence