Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0134 and Shew_0135 overlap by 1 nucleotides Shew_0135 and Shew_0136 are separated by 162 nucleotides
Shew_0134: Shew_0134 - tRNA 2-selenouridine synthase (RefSeq), at 143,555 to 144,673
_0134
Shew_0135: Shew_0135 - selenide, water dikinase (RefSeq), at 144,673 to 145,716
_0135
Shew_0136: Shew_0136 - stearoyl-CoA 9-desaturase (RefSeq), at 145,879 to 146,985
_0136
Position (kb) 144
145
146 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 143.704 kb on + strand, within Shew_0134 at 143.804 kb on - strand, within Shew_0134 at 143.973 kb on + strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.986 kb on - strand, within Shew_0134 at 144.048 kb on + strand, within Shew_0134 at 144.053 kb on + strand, within Shew_0134 at 144.255 kb on - strand, within Shew_0134 at 144.265 kb on + strand, within Shew_0134 at 144.619 kb on + strand at 144.627 kb on - strand at 144.684 kb on - strand at 144.963 kb on + strand, within Shew_0135 at 144.971 kb on - strand, within Shew_0135 at 144.971 kb on - strand, within Shew_0135 at 145.396 kb on + strand, within Shew_0135 at 145.520 kb on + strand, within Shew_0135 at 145.644 kb on - strand at 145.703 kb on - strand at 145.746 kb on + strand at 145.770 kb on - strand at 145.770 kb on - strand at 145.770 kb on - strand at 145.991 kb on + strand, within Shew_0136 at 146.102 kb on + strand, within Shew_0136 at 146.103 kb on + strand, within Shew_0136 at 146.168 kb on + strand, within Shew_0136 at 146.187 kb on + strand, within Shew_0136 at 146.359 kb on - strand, within Shew_0136 at 146.374 kb on - strand, within Shew_0136 at 146.374 kb on - strand, within Shew_0136 at 146.407 kb on - strand, within Shew_0136 at 146.449 kb on + strand, within Shew_0136 at 146.498 kb on - strand, within Shew_0136
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 143,704 + Shew_0134 0.13 +0.7 143,804 - Shew_0134 0.22 -0.7 143,973 + Shew_0134 0.37 -1.9 143,981 - Shew_0134 0.38 +2.9 143,981 - Shew_0134 0.38 +0.5 143,981 - Shew_0134 0.38 +1.0 143,981 - Shew_0134 0.38 +0.4 143,986 - Shew_0134 0.39 -1.2 144,048 + Shew_0134 0.44 +1.6 144,053 + Shew_0134 0.45 +0.9 144,255 - Shew_0134 0.63 -0.7 144,265 + Shew_0134 0.63 +0.1 144,619 + +1.4 144,627 - +0.6 144,684 - -1.2 144,963 + Shew_0135 0.28 -1.5 144,971 - Shew_0135 0.29 -1.5 144,971 - Shew_0135 0.29 +0.1 145,396 + Shew_0135 0.69 +0.0 145,520 + Shew_0135 0.81 +0.3 145,644 - -4.0 145,703 - -0.0 145,746 + +0.2 145,770 - -1.0 145,770 - +1.0 145,770 - +0.2 145,991 + Shew_0136 0.10 +0.8 146,102 + Shew_0136 0.20 -0.2 146,103 + Shew_0136 0.20 -0.9 146,168 + Shew_0136 0.26 -1.5 146,187 + Shew_0136 0.28 -0.5 146,359 - Shew_0136 0.43 +1.9 146,374 - Shew_0136 0.45 +2.3 146,374 - Shew_0136 0.45 -0.5 146,407 - Shew_0136 0.48 +0.4 146,449 + Shew_0136 0.51 +1.8 146,498 - Shew_0136 0.56 -0.0
Or see this region's nucleotide sequence