Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0117 and Shew_0118 are separated by 12 nucleotides Shew_0118 and Shew_0119 overlap by 1 nucleotides Shew_0119 and Shew_0120 are separated by 172 nucleotides Shew_0120 and Shew_0121 are separated by 40 nucleotides
Shew_0117: Shew_0117 - protoheme IX farnesyltransferase (RefSeq), at 128,380 to 129,282
_0117
Shew_0118: Shew_0118 - cytochrome oxidase assembly (RefSeq), at 129,295 to 130,278
_0118
Shew_0119: Shew_0119 - hypothetical protein (RefSeq), at 130,278 to 130,829
_0119
Shew_0120: Shew_0120 - hypothetical protein (RefSeq), at 131,002 to 131,757
_0120
Shew_0121: Shew_0121 - hypothetical protein (RefSeq), at 131,798 to 131,974
_0121
Position (kb)
130
131 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 129.312 kb on + strand at 129.335 kb on - strand at 129.392 kb on - strand at 129.506 kb on + strand, within Shew_0118 at 129.514 kb on - strand, within Shew_0118 at 129.514 kb on - strand, within Shew_0118 at 129.514 kb on - strand, within Shew_0118 at 129.611 kb on + strand, within Shew_0118 at 129.681 kb on + strand, within Shew_0118 at 129.797 kb on - strand, within Shew_0118 at 129.993 kb on + strand, within Shew_0118 at 130.029 kb on + strand, within Shew_0118 at 130.201 kb on - strand at 130.244 kb on - strand at 130.246 kb on - strand at 130.256 kb on - strand at 130.293 kb on - strand at 130.485 kb on - strand, within Shew_0119 at 130.509 kb on - strand, within Shew_0119 at 130.536 kb on + strand, within Shew_0119 at 130.616 kb on + strand, within Shew_0119 at 130.646 kb on - strand, within Shew_0119 at 131.161 kb on + strand, within Shew_0120 at 131.258 kb on - strand, within Shew_0120 at 131.404 kb on - strand, within Shew_0120 at 131.452 kb on - strand, within Shew_0120 at 131.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 129,312 + +1.5 129,335 - -0.2 129,392 - -1.8 129,506 + Shew_0118 0.21 -1.5 129,514 - Shew_0118 0.22 -1.2 129,514 - Shew_0118 0.22 -1.9 129,514 - Shew_0118 0.22 -0.8 129,611 + Shew_0118 0.32 -0.3 129,681 + Shew_0118 0.39 -1.5 129,797 - Shew_0118 0.51 -3.1 129,993 + Shew_0118 0.71 -3.2 130,029 + Shew_0118 0.75 -2.2 130,201 - -1.0 130,244 - -2.3 130,246 - +0.1 130,256 - -1.8 130,293 - -0.5 130,485 - Shew_0119 0.38 -0.3 130,509 - Shew_0119 0.42 -0.8 130,536 + Shew_0119 0.47 -1.0 130,616 + Shew_0119 0.61 -0.6 130,646 - Shew_0119 0.67 -0.1 131,161 + Shew_0120 0.21 +0.2 131,258 - Shew_0120 0.34 -0.7 131,404 - Shew_0120 0.53 +0.1 131,452 - Shew_0120 0.60 -1.6 131,803 - -1.0
Or see this region's nucleotide sequence