Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0073 and Shew_0074 are separated by 99 nucleotides Shew_0074 and Shew_0075 overlap by 4 nucleotides Shew_0075 and Shew_0076 are separated by 292 nucleotides Shew_0076 and Shew_0077 are separated by 21 nucleotides
Shew_0073: Shew_0073 - coproporphyrinogen III oxidase (RefSeq), at 86,819 to 88,162
_0073
Shew_0074: Shew_0074 - no description, at 88,262 to 88,885
_0074
Shew_0075: Shew_0075 - hypothetical protein (RefSeq), at 88,882 to 89,097
_0075
Shew_0076: Shew_0076 - antibiotic biosynthesis monooxygenase (RefSeq), at 89,390 to 89,677
_0076
Shew_0077: Shew_0077 - GCN5-related N-acetyltransferase (RefSeq), at 89,699 to 90,652
_0077
Position (kb)
88
89 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 87.296 kb on - strand, within Shew_0073 at 87.296 kb on - strand, within Shew_0073 at 87.302 kb on - strand, within Shew_0073 at 87.608 kb on + strand, within Shew_0073 at 87.641 kb on + strand, within Shew_0073 at 87.801 kb on + strand, within Shew_0073 at 88.106 kb on + strand at 88.114 kb on - strand at 88.122 kb on + strand at 88.167 kb on + strand at 88.526 kb on + strand, within Shew_0074 at 88.665 kb on + strand, within Shew_0074 at 88.665 kb on + strand, within Shew_0074 at 88.673 kb on - strand, within Shew_0074 at 88.673 kb on - strand, within Shew_0074 at 88.673 kb on - strand, within Shew_0074 at 88.673 kb on - strand, within Shew_0074 at 88.755 kb on + strand, within Shew_0074 at 88.847 kb on + strand at 89.081 kb on - strand at 89.113 kb on - strand at 89.291 kb on + strand at 89.299 kb on - strand at 89.531 kb on + strand, within Shew_0076 at 89.567 kb on + strand, within Shew_0076 at 89.572 kb on + strand, within Shew_0076 at 89.671 kb on + strand at 89.671 kb on + strand at 89.671 kb on + strand at 89.840 kb on - strand, within Shew_0077
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 87,296 - Shew_0073 0.35 +0.2 87,296 - Shew_0073 0.35 +0.2 87,302 - Shew_0073 0.36 -2.9 87,608 + Shew_0073 0.59 -0.1 87,641 + Shew_0073 0.61 -0.9 87,801 + Shew_0073 0.73 +0.3 88,106 + -0.2 88,114 - +0.3 88,122 + -0.2 88,167 + +0.4 88,526 + Shew_0074 0.42 -0.3 88,665 + Shew_0074 0.65 -0.2 88,665 + Shew_0074 0.65 -0.1 88,673 - Shew_0074 0.66 +2.5 88,673 - Shew_0074 0.66 -0.3 88,673 - Shew_0074 0.66 -0.7 88,673 - Shew_0074 0.66 -1.7 88,755 + Shew_0074 0.79 +0.2 88,847 + -1.4 89,081 - +0.2 89,113 - -1.5 89,291 + +0.5 89,299 - +0.7 89,531 + Shew_0076 0.49 -0.6 89,567 + Shew_0076 0.61 +0.4 89,572 + Shew_0076 0.63 -0.0 89,671 + -1.3 89,671 + +1.6 89,671 + -3.2 89,840 - Shew_0077 0.15 +1.6
Or see this region's nucleotide sequence